H6916

Macrophage Colony-Stimulating Factor human

≥95% (SDS-PAGE), recombinant, expressed in HEK 293 cells, lyophilized powder, suitable for cell culture

• Sinonimo/i: MCSF
• Numero MDL: MFCD00166591
• Codice UNSPSC: 12352202
• NACRES: NA.77

Proprietà

• product name: Macrophage Colony-Stimulating Factor human, M-CSF, recombinant, expressed in HEK 293 cells, suitable for cell culture
• Origine biologica: human
• Livello qualitativo: 100
• Ricombinante: expressed in HEK 293 cells
• Saggio: ≥95% (SDS-PAGE)
• Forma fisica: lyophilized powder
• Potenza: ≤4 ng/mL EC₅₀
• Qualità: endotoxin tested
• PM: dimer 35-40 kDa (glycosylated)
• Confezionamento: pkg of 10 μg
• Produttore/marchio commerciale: HumanZyme
• tecniche: cell culture | mammalian: suitable
• N° accesso UniProt: P09603
• Temperatura di conservazione: −20°C
• Informazioni sul gene: human ... CSF1(1435)

Descrizione

Descrizione generale

M-CSF is encoded by the gene mapped to human chromosome 1p13.3. It is a glycosylated polypeptide, which exists as a homodimer. M-CSF is produced by macrophage, fibroblasts, lymphocyte and endothelial cells.

Azioni biochim/fisiol

Macrophage CSF (M-CSF) as a growth factor, stimulates the proliferation and differentiation of monocyte/macrophage-lineage cells. It also boosts antibody-dependent, cell-mediated cytotoxicity by monocytes and macrophages. In addition, human M-CSF inhibits bone resorption by osteoclasts. It might also play a crucial role in atherosclerosis. Overexpression of the gene has been observed in Paget′s disease of bone (PDB).

Stato fisico

Lyophilized from a 0.2 μm filtered solution of 1x PBS.

Risultati analitici

The specific activity was determined by the dose-dependent stimulation of the proliferation of murine M-NFS-60 cells (mouse myeloid Leukemia indicator cell line).

Citazione

1. Mazur, E.M., and Cohen, J.L., Clin. Pharmacol. Ther., 46, 250 (1989).
2. Morstyn, G., and Burgess, A.W., Cancer Res., 48, 5624 (1988).
3. Metcalf, D., Blood, 67, 257 (1986).
4. Mufson, R.A., et al., Cell. Immunol., 119, 182 (1989).
5. Hattersley, G., et al., J. Cell Physiol., 137, 199 (1988).

Note legali

HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable