H0654

Hygromycin B solution from Streptomyces hygroscopicus

≥60% (HPLC), 45-60 mg/mL in H₂O

• Sinonimo/i: Hygromycin B, Hygrovetine
• Formula empirica (notazione di Hill): C₂₀H₃₇N₃O₁₃
• Numero CAS: 31282-04-9
• Peso molecolare: 527.52
• Beilstein: 6755837
• Numero MDL: MFCD06795479
• Codice UNSPSC: 51102829
• ID PubChem: 24895410
• NACRES: NA.85

Proprietà

• Origine biologica: Streptomyces hygroscopicus
• Livello qualitativo: 200
• Sterilità: 0.22 μm filtered
• Concentrazione: ≥60% (HPLC); 45-60 mg/mL in H₂O
• Colore: brown-yellow; light orange to dark orange; light yellow to dark yellow
• Spettro attività antibiotica: viruses
• Modalità d’azione: protein synthesis | interferes
• Temperatura di conservazione: 2-8°C
• Stringa SMILE: CN[C@H]1C[C@@H](N)[C@H](O)[C@@H](O[C@@H]2O[C@H](CO)[C@H](O)[C@@H]3OC4(O[C@H]([C@H](N)CO)[C@H](O)[C@H](O)[C@H]4O)O[C@H]23)[C@@H]1O
• InChI: 1S/C20H37N3O13/c1-23-7-2-5(21)9(26)15(10(7)27)33-19-17-16(11(28)8(4-25)32-19)35-20(36-17)18(31)13(30)12(29)14(34-20)6(22)3-24/h5-19,23-31H,2-4,21-22H2,1H3/t5-,6-,7+,8-,9+,10-,11+,12-,13+,14-,15-,16+,17+,18-,19+,20+/m1/s1; GRRNUXAQVGOGFE-XKIAHZFYSA-N

Descrizione

Descrizione generale

Chemical structure: aminoglycoside

Applicazioni

Hygromycin B is an aminoglycoside antibiotic isolated from Streptomyces hygroscopicus. It has been used to study protein synthesis at the level of the 70S ribosome translocation and mRNA template misreading, as an antiviral agent by selectively penetrating cells rendered permeable by virus infection and inhibiting translation, and as a selection agent for hygromycin resistance gene transformed cells. It is recommended for use as a selection agent at 100-800 μg/mL, specifically at 100 μg/mL for prokaryotes, 200 μg/mL for lower eukaryotes and 150-400 μg/mL for higher eukaryotes.

Azioni biochim/fisiol

Mode of action: The product acts by inhibiting protein synthesis by inducing the misreading of the m-RNA template in the prokaryote, with the potency to inhibit translation.

Antimicrobial Spectrum: Hygromycin B acts against bacteria, fungi and higher eukaryotic cells.

Avvertenza

Hygromycin B products should be stored as supplied at 2-8°C, and the dry solid is stable for at least 5 years if stored at 2-8°C. It is stable at 37°C for 30 days.

Nota sulla preparazione

This product is a solution that comes =60% (HPAE) and 45-60 mg/mL in H₂O.

Informazioni sulla sicurezza

• Pittogrammi: GHS06
• Avvertenze: Danger
• Indicazioni di pericolo: H301 + H311,H330
• Consigli di prudenza: P260 - P264 - P270 - P280 - P302 + P352 + P312 - P304 + P340 + P310
• Classi di pericolo: Acute Tox. 1 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 3 Oral
• Codice della classe di stoccaggio: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Dispositivi di protezione individuale: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter