GE17-0440-01

Con-A Sepharose^™ 4B

Cytiva 17-0440-01, pack of 100 mL

• Sinonimo/i: Concanavalin A Sepharose
• Codice UNSPSC: 41106500
• NACRES: NA.56

Proprietà

• ligand: concanavalin A (con A)
• Confezionamento: pack of 100 mL
• Produttore/marchio commerciale: Cytiva 17-0440-01
• Condizioni di stoccaggio: (0.1 M acetate buffer pH 6, 1 M NaCl, 1 mM CaCl2, 1 mM MnCl2 and 1 mM MgCl, 20% Ethanol)
• Matrice: 4% agarose
• Dimensione particelle: 45-165 μm
• Diametro medio: 90 μm
• cleaning in place: 4-9
• working range: 4-6
• Temperatura di conservazione: 2-8°C

Descrizione

Descrizione generale

Con A Sepharose^™ 4B with immobilized Concanavalin A is an affinity chromatography media for capture of glycosylated biomolecules including glycoproteins and glycolipids. Stronger affinity for α-D-glucose than α-D-mannose.

Con A Sepharose^™ is Concanavalin A coupled to Sepharose^™ 4B by the cyanogen bromide method. Concanavalin A (Con A) is a tetrameric metalloprotein isolated from Canavalia ensiformis (jack bean). Con A binds molcules containing α-D-mannopyranosyl, α-D-glucopyranosyl and sterically related residues. The binding sugar requires the presence of C-3, C-4 and C-5 hydroxyl groups for reaction with Con A. Con A coupled to Sepharose^™ is routinely used for separation and purification of glycoproteins, polysaccharides and glycolipids.

Caratteristiche e vantaggi

• Concanavalin A (Con A) binds molecules that contain α-D-mannose, α-D-glucose, and sterically related residues with available C-3, C-4, or C-5 hydroxyl groups
• Group-specific adsorbent for molecules containing sugars.
• Highly suitable for isolation of cell surface glycoproteins from detergent-solubilized membranes
• Concanavalin A (Con A) binds molecules that contain α-D-mannose, α-D-glucose and sterically related residues with available C-3, C-4, or C-5 hydroxyl groups
• Highly suitable for isolation of cell surface glycoproteins from detergent-solubilized membranes
• Con A coupled to Sepharose^™ 4B via cyanogen bromide activation

Stoccaggio e stabilità

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Risultati analitici

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Note legali

Sepharose is a trademark of Cytiva

Informazioni sulla sicurezza

• Pittogrammi: GHS02
• Avvertenze: Warning
• Indicazioni di pericolo: H226
• Consigli di prudenza: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Codice della classe di stoccaggio: 3 - Flammable liquids