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D6442

Sigma-Aldrich

JumpStart™ Taq ReadyMix™ for High Throughput Quantitative PCR

Ready-to-use 2x mix for qPCR with ROX

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About This Item

Codice UNSPSC:
41106300

Forma fisica

liquid

impiego

sufficient for 20 reactions
 sufficient for 2000 reactions
 sufficient for 400 reactions

Caratteristiche

dNTPs included
hotstart

Concentrazione

2.5 units/reaction (50 μL reaction volume)

tecniche

qPCR: suitable

Colore

colorless

input

purified DNA

applicazioni

agriculture

Compatibilità

for use with ABI 5700
for use with ABI 7000
for use with ABI 7300
for use with ABI 7500 Fast
for use with ABI 7500
for use with ABI 7700
for use with ABI 7900 Fast
for use with ABI 7900 HT
for use with ABI 7900
for use with ABI StepOne
for use with ABI StepOnePlus
for use with ABI ViiA 7
for use with Bio-Rad CFX384
for use with Bio-Rad CFX96
for use with Bio-Rad MJ Chromo4
for use with Bio-Rad MJ Opticon 2
for use with Bio-Rad MJ Opticon Cepheid SmartCycler
for use with Bio-Rad MJ Opticon
for use with Bio-Rad MiniOpticon
for use with Eppendorf® Mastercycler ep realplex2 s
for use with Eppendorf® Mastercycler ep realplex
for use with Illumina Eco qPCR
for use with Qiagen Corbett Rotor-Gene 3000
for use with Qiagen Corbett Rotor-Gene 6000
for use with Qiagen Corbett Rotor-Gene Q
for use with Roche LightCycler 480
for use with Strategene Mx3000P
for use with Strategene Mx3005P
for use with Strategene Mx4000

Metodo di rivelazione

probe-based

Condizioni di spedizione

wet ice

Temperatura di conservazione

−20°C

Categorie correlate

Descrizione generale

JumpStart™ Taq ReadyMix™ For High Throughput Quantitative PCR combines the performance enhancements of JumpStart Taq Antibody for hot start PCR with the convenience of an easy-to-use reaction mixture that incorporates the internal reference dye for ABI and other real time instrument applications. This 2× concentrate master mix contains JumpStart Taq DNA polymerase, 99% pure deoxynucleotides and reaction buffer. Simply add an equal volume of the 2× ReadyMix to a 2× mixture DNA template, primers and fluorescent probe.
To prepare a typical qPCR reaction, mix 25 μL of JumpStart ReadyMix Taq, fluorescent probe, desired amount of magnesium chloride (above 1.5 mM final concentration), template DNA, and primers in a final volume of 50 μL. Reaction volumes can be scaled down, if desired.

Applicazioni

JumpStart™ Taq ReadyMix™ for High Throughput Quantitative PCR has been used:
  • For quantitative polymerase chain reaction (qPCR) of cDNA template
  • As a component of PCR reaction mix for amplification of DNA extracted from peripheral blood leucocytes
  • For qPCR amplifications using SYBR
  • For multiplex PCR
  • For reduction of primer dimers

Caratteristiche e vantaggi

  • JumpStart™ Taq DNA polymerase prevents amplification of non-specific products, resulting in increased efficiency and higher target yield
  • When performing large number of PCR reactions, JumpStart™ Taq ReadyMix™ reduces preparation time and the risk of contamination from multiple pipetting steps
  • This master mix allows consistent batch-to-batch and reaction-to-reaction performance
  • Internal Reference Dye is provided for reaction normalization

Confezionamento

Default reaction volume is 50 μL

20RXN is packaged as 1 X 500 μL
400RXN is packaged as 1 X 10 mL
2000RXN is packaged as 1 X 50 mL

Quantità

Use 2.5 units per reaction.

Note legali

Eppendorf is a registered trademark of Eppendorf AG
JumpStart is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Avvertenze

Danger

Classi di pericolo

Acute Tox. 4 Inhalation - Aquatic Chronic 2 - Eye Dam. 1 - Ox. Liq. 3 - Skin Irrit. 2 - Skin Sens. 1

Codice della classe di stoccaggio

5.1B - Oxidizing hazardous materials

Classe di pericolosità dell'acqua (WGK)

WGK 3

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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