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KCQS02

Sigma-Aldrich

KiCqStart® SYBR® Green qPCR ReadyMix

with ROX for ABI instruments

Sinonimo/i:

qPCR master mix, sybr green qPCR

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About This Item

Codice UNSPSC:
41106300
NACRES:
NA.55

Prezzi e disponibilità al momento non sono disponibili

Forma fisica

liquid

impiego

sufficient for 1250 reactions
sufficient for 250 reactions
sufficient for 5000 reactions

Caratteristiche

dNTPs included
hotstart

Condizioni di stoccaggio

protect from light

tecniche

qPCR: suitable

Colore

colorless

input

purified DNA

Compatibilità

for use with ABI 5700
for use with ABI 7000
for use with ABI 7300
for use with ABI 7700
for use with ABI 7900 HT Fast
for use with ABI 7900 HT
for use with ABI 7900
for use with ABI StepOne
for use with ABI StepOnePlus

Metodo di rivelazione

SYBR® Green

Condizioni di spedizione

dry ice

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Descrizione generale

KiCqStart SYBR Green qPCR ReadyMix is a 2X concentrated, ready-to-use master mix that contains all components, except primers and template, for real-time quantitative PCR (qPCR) This unique combination of proprietary buffer, stabilizers, and Hot-Start Taq DNA polymerase delivers maximum PCR efficiency, sensitivity, specificity and robust fluorescent signal using fast, or conventional, cycling protocols with SYBR Green qPCR.

Highly specific amplification is crucial to successful qPCR with SYBR Green I dye technology because this dye binds to and detects any dsDNA generated during amplification. Hot-Start Taq DNA polymerase is antibody mediated to be inactive prior to the initial PCR denaturation step.

Applicazioni

KiCqStart® SYBRSYB® Green qPCR ReadyMix has been used:

  • in the amplification and quantification of cDNA reverse transcribed from RNA extracted from mice brain samples in a 2-step RT-qPCR assay[1]
  • to analyze DNA purified by ChIP technique[2]
  • to perform gene expression analysis[3]
  • in amplification of RNA isolated from hearts of adult TL wild-type fish by quantitative real-time polymerase chain reaction (PCR) [4]
PCR applications:
  • Gene expression
  • DNA quantification
  • CHiP

Caratteristiche e vantaggi

  • Assay results in as little as 33 minutes
  • Highly efficient and sensitive real-time PCR results
  • Little/no optimization required

Componenti

2X reaction buffer containing optimized concentrations of MgCl2, dNTPs, (dATP, dCTP, dGTP, dTTP), KiCqStart Taq DNA Polymerase, SYBR Green dye, ROX Reference Dye (for 580-585 nm excitation), and stabilizers.

packaging:
250 reactions* = 2 X 1.25 mL tubes
1250 reactions* = 10 X 1.25 mL tubes
5000 reactions* = 1 X 50 mL tube
*number of reactions based on a 20uL volume

Altre note

Storage Conditions:
KiCqStart SYBR Green qPCR ReadyMix is stable for 1 year when stored in a constant temperature freezer at -20°C, protected from light. For convenience, it may be stored unfrozen at +2 to +8°C for up to 6 months. After thawing, mix thoroughly before using. Repeated freezing and thawing of the product is not recommended. However, the product demonstrated no loss of performance after 20 freeze-thaw cycles or 2 months at +20°C.

Note legali

Applied Biosystems is a registered trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
KiCqStart is a registered trademark of QIAGEN Beverly Inc.
ROX is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
SYBR is a registered trademark of Life Technologies
StepOne is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
StepOnePlus is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries

Raccomandato

N° Catalogo
Descrizione
Determinazione del prezzo

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

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Articoli

After a traditional PCR has been completed, the PCR/qPCR data analysis is conducted by resolution through an agarose gel or, more recently, through a capillary.

PCR assay guide navigates you through primer validation and other assay optimization factors to ensure high sensitivity and specificity for optimum DNA/ RNA quantification.

Real-time polymerase chain reaction allows researchers to estimate the quantity of starting material in a sample. It has a much wider dynamic range of analysis than conventional PCR

Protocolli

Quantitative PCR protocol using SYBR Green reagents. Procedure supports most qPCR instruments.

Analysis of gene expression data requires a stable reference or loading control. This reference is usually one or more reference genes.

Once an assay has been optimized, it is important to verify the reaction efficiency. This information is important when reporting and comparing assays. In this example protocol, the assay efficiency is compared over a wide and narrow dynamic range of cDNA concentrations.

Gradient PCR for assay optimization is to determine the optimum annealing temperature (Ta) of the primers by testing identical reactions containing a fixed primer concentration, across a range of annealing temperatures.

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