D5319
Deoxyribonuclease I bovine
recombinant, expressed in Pichia pastoris, buffered aqueous glycerol solution, ≥5,000 units/mg protein
Sinonimo/i:
DNAse I, Deoxyribonucleate 5′-oligonucleotido-hydrolase
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About This Item
Numero MDL:
Codice UNSPSC:
12352204
NACRES:
NA.54
Prodotti consigliati
Origine biologica
bovine
Livello qualitativo
Ricombinante
expressed in Pichia pastoris
Saggio
≥95%
Forma fisica
buffered aqueous glycerol solution
Attività specifica
≥5,000 units/mg protein
PM
~39 kDa
tecniche
DNA extraction: suitable
Compatibilità
suitable for molecular biology
applicazioni
diagnostic assay manufacturing
Attività estranea
RNAse and protease, free
Condizioni di spedizione
dry ice
Temperatura di conservazione
−20°C
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Categorie correlate
Applicazioni
DNAse I from Sigma was used to treat nuclear lysate to obtain single nucleosomes in a study. The enzyme has also been for the preparation and harvest of mice mammary glands.
Deoxyribonuclease I bovine has been used in a study to compare the initial actions of spleen deoxyribonuclease and pancreatic deoxyribonuclease. Deoxyribonuclease I bovine has also been used in a study to investigate deoxythymidine 3′, 5′-di-p-nitrophenyl phosphate as a synthetic substrate for bovine pancreatic deoxyribonuclease.
Used for the removal of DNA from protein samples.
Azioni biochim/fisiol
DNase I is an endonuclease that acts on phosphodiester bonds (adjacent to pyrimidines) to produce polynucleotides with terminal 5′-phosphates. The pH optimum is found to be between 7 and 8. Divalent cations such as Mn2+, Ca2+, Co2+, and Zn2+ are activators of the enzyme. A concentration of 5 mM Ca2+ stabilizes the enzyme against proteolytic digestion. 2-Mercaptoethanol, chelators, sodium dodecyl sulfate (SDS) and actin are known to inhibit the enzyme activity.
Digests single- and double-stranded DNA to a mixture of mono- and oligonucleotides carrying 5′ phosphates and 3′ OH termini. This catalytic activity is divalent ion-dependent. In the presence of Mg2+, DNase I hydrolyzes each strand of double-stranded DNA randomly and independently. In the presence of Mn2+, both strands can be cleaved.
Caratteristiche e vantaggi
- RNA purification by removing DNA
- Prepare DNA for nick translation1
- Footprinting assays to determine DNA-protein interactions2
Definizione di unità
One unit will produce a ΔA260 of 0.001 per min per mL reaction mixture using calf thymus DNA at pH 5.0 and 25°C
Stato fisico
Supplied as a solution in 4 mg/ml glycine pH 5.0, 5 mM calcium acetate and 50% glycerol
Nota sulla preparazione
Produced without using any animal cells or animal derived materials.
Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
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