CE0050
CelLytic™ MEM Protein Extraction Kit
Sinonimo/i:
Membrane protein extraction kit
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About This Item
Codice UNSPSC:
41116134
NACRES:
NA.56
Prodotti consigliati
tecniche
protein extraction: suitable
Livello qualitativo
Condizioni di spedizione
wet ice
Temperatura di conservazione
−20°C
Descrizione generale
CelLytic™ MEM Protein Extraction Kit offers a fast and convenient method to isolate hydrophobic and raft microdomain associated proteins from cells. The method is based on phase separation and does not require cell membrane isolation. The separated proteins can be used for further experiments such as SDS-PAGE, Western blotting, dot blotting, and immunoprecipitation. The kit has been tested on, but not limited to, HeLa, HEK-293, NIH 3T3, COS and CHO cell lines.
Membrane proteins make up around 20-30% of an organism′s genome and serve as cellular gatekeepers, regulators, and sensors. They have diverse cellular functions, such as shielding the cell from external toxins, being the starting point of intracellular signaling cascades, and retaining critical ion concentrations.
Applicazioni
CelLytic™ MEM Protein Extraction Kit has been used for de novo lipogenesis measurements using hepatocytes and to extract membrane proteins for western blotting.
Compatibilità
Sufficient reagents supplied for 80 tests.
Note legali
CelLytic is a trademark of Sigma-Aldrich Co. LLC
Solo come componenti del kit
N° Catalogo
Descrizione
- Lysis and Separation Buffer 50 mL
- Wash Buffer for CelLytic MEM 50 mL
- Sodium Chloride, 4M Solution 1.5 mL
I componenti del kit sono disponibili anche separatamente
N° Catalogo
Descrizione
SDS
- P8340Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution 1 mLSDS
Avvertenze
Warning
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Eye Irrit. 2 - Skin Irrit. 2
Codice della classe di stoccaggio
10 - Combustible liquids
Punto d’infiammabilità (°F)
188.6 °F - closed cup
Punto d’infiammabilità (°C)
87 °C - closed cup
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