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Merck
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Documenti fondamentali

70584-M

Millipore

BugBuster® Protein Extraction Reagent

Sinonimo/i:

Protein Extraction Reagent, Protein Extraction Solution

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About This Item

Codice UNSPSC:
41106516
NACRES:
NA.32

Stato

liquid

Livello qualitativo

100

Produttore/marchio commerciale

Novagen®

Condizioni di stoccaggio

OK to freeze
avoid repeated freeze/thaw cycles

Temperatura di conservazione

10-30°C

Descrizione generale

BugBuster® Protein Extraction Reagent is formulated for gentle disruption of Escherichia coli cell wall to liberate active proteins. It provides a simple, rapid, low-cost alternative to mechanical methods such as French Press or sonication for releasing expressed target protein in preparation for purification or other applications. The proprietary formulation utilizes a tris-buffer-based mixture of non-ionic and zwitterionic detergents that is capable of cell wall perforation without denaturing the protein. BugBuster is also useful for the preparation of high-purity inclusion bodies for subsequent purification in instances where expressed proteins are insoluble.

Applicazioni

BugBuster® Protein Extraction Reagent has been used:
  • to lyse Escherichia coli BLR (DE3) cells for β-glucosidase (BglB) production and purification
  • as a cell lysis buffer to treat the synechocystis PCC6803 transconjugants and measure the yellow fluorescent protein (YFP) fluorescence
  • in lysis buffer for purification of glutathione S-transferase- heat shock protein family A (Hsp70) member 8 (GST- HSPA8)

Attenzione

Toxicity: Standard Handling (A)

Note legali

BUGBUSTER is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Patricia Armshaw et al.
Biotechnology for biofuels, 8, 201-201 (2015-12-03)
The use of photosynthetic autotrophs and in particular the model organism Synechocystis PCC6803 is receiving much attention for the production of sustainable biofuels and other economically useful products through metabolic engineering. Optimisation of metabolic-engineered organisms for high-level sustained production of
Tiejian Nie et al.
Autophagy, 17(10), 2923-2938 (2021-05-11)
As a highly dynamic organelle, mitochondria undergo constant fission and fusion to change their morphology and function, coping with various stress conditions. Loss of the balance between fission and fusion leads to impaired mitochondria function, which plays a critical role

Articoli

Citation Spotlight: BugBuster® Protein Extraction Reagent for Efficient Protein Extraction from Bacterial Pathogens

Citation Spotlight: BugBuster® Protein Extraction Reagent for Efficient Protein Extraction from Bacterial Pathogens

BugBuster® and Benzonase® Reagents are the Clear Solutions to Simple, Efficient Extraction of E. coli Proteins

This article shows the use of BugBuster® and Benzonase® as protein purification tools to extract recombinant proteins from E. coli and to reduce the viscosity of the extract.

Contenuto correlato

Lysis & Protein Extraction

An overview of cell lysis and protein extraction methods including detergent solubilization, freeze-thaw lysis, osmotic shock, sonication, enzymatic cell lysis, and mechanical disruption techniques such as Dounce, Polytron, and mortar and pestle homogenization.

Lysis & Protein Extraction

An overview of cell lysis and protein extraction methods including detergent solubilization, freeze-thaw lysis, osmotic shock, sonication, enzymatic cell lysis, and mechanical disruption techniques such as Dounce, Polytron, and mortar and pestle homogenization.

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