PE0220
Plant Hydrophobic Protein Extraction Kit
Suitable for any plant species or tissue
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About This Item
Codice UNSPSC:
41105500
NACRES:
NA.56
Prodotti consigliati
Livello qualitativo
Temperatura di conservazione
−20°C
Descrizione generale
The Plant Hydrophobic Protein Extraction Kit is designed specifically for use in plant bioscience to extract a qualitative sample of hydrophobic proteins from any type of plant species or tissue. The protocol does not require any ultracentrifugation or aqueous polymer two-phase partitioning (APTP). The kit includes three reagents, a plant specific protease inhibitor cocktail, a specially formulated reagent to extract hydrophilic proteins (Plant Protein Extraction Reagent Type 1), and a new chaotropic reagent with increased solubilizing power to extract more hydrophobic proteins (C0356, Protein Extraction Reagent Type 4). The protease inhibitor cocktail is a mixture of protease inhibitors with broad specificity for the inhibition of serine, cysteine, aspartic, metalloproteases, and aminopeptidases. It contains pepstatin A, E-64, leupeptin, 4-(2-aminoethyl)benzenesulfonyl fluoride (AEBSF), bestatin, and 1,10-phenanthroline. The cocktail has been demonstrated to be highly effective in preventing protein degradation during the extraction process.
Following removal of polyphenolics, tannins, and other interfering substances, ground plant tissue, fresh or frozen, is resuspended in a specially formulated reagent to extract hydrophilic proteins. After sequential extractions of the hydrophilic proteins, the chaotropic reagent is used to extract hydrophobic membrane bound proteins. Plant debris is pelleted by centrifugation and the hydrophobic protein extract is collected. The end result is a qualitative hydrophobic protein sample, ready for downstream proteomic analysis.
Following removal of polyphenolics, tannins, and other interfering substances, ground plant tissue, fresh or frozen, is resuspended in a specially formulated reagent to extract hydrophilic proteins. After sequential extractions of the hydrophilic proteins, the chaotropic reagent is used to extract hydrophobic membrane bound proteins. Plant debris is pelleted by centrifugation and the hydrophobic protein extract is collected. The end result is a qualitative hydrophobic protein sample, ready for downstream proteomic analysis.
Quantità
Sufficient for preparation of 20 separate extractions from plant tissue samples of 10-250mg
Avvertenze
Warning
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Repr. 2
Codice della classe di stoccaggio
10 - Combustible liquids
Punto d’infiammabilità (°F)
185.0 °F
Punto d’infiammabilità (°C)
85 °C
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Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 879(22), 2179-2183 (2011-06-22)
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Journal of biomolecular techniques : JBT, 20(4), 201-215 (2009-09-02)
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