A375-C5 Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• Codice UNSPSC: 41106514

Proprietà

• Origine biologica: human skin
• Descrizione: Human malignant melanoma IL-1 insensitive
• Confezionamento: tube of 5 μg 97011320-DNA-5UG; pkg of vial of cells 97011320-1VL
• Modalità di accrescimento: Adherent
• Cariotipo: Not specified
• Morfologia: Epithelial-like
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Human malignant melanoma IL-1 insensitive

Descrizione della linea cellulare

The cell line A375-C5 is unresponsive to the anti-proliferative effect of IL-1. It has been derived from the parental line A373 (Sigma catalogue no. 88113005) which was subjected to cloning by limiting dilution. This method resulted in the establishment of A375-C5 and an IL-1 sensitive clone, A375-C6 (Sigma catalogue no. 97011321). The unresponsiveness of A375-C5 is probably due to an IL-1 receptor deficiency. The cells have been eradicated from mycoplasma at ECACC.

Applicazioni

IL-1 resistance studies, receptor studies

Terreno di coltura

RPMI 1640 + 2mM Glutamine + 10% FBS

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:5 using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C

Altre note

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