FM-95

from human skin, 13012425

• Codice UNSPSC: 41106514

Proprietà

• Origine biologica: human skin
• Descrizione: Human melanoma, metastasis
• Modalità di accrescimento: Adherent
• Morfologia: Fibroblastic
• tecniche: cell culture | mammalian: suitable
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Descrizione della linea cellulare

FM-95 (ESTDAB-034) was established from a metastatic cutaneous malignant melanoma of a male patient. The FM-95 cell line is one of more than 170 characterized melanoma cell lines collected during the ESTDAB project (European Searchable Tumour Line Database) part of the European Commissions fifth framework infrastructures programme (contract no. QLRI-CT-2001-01325). ESTDAB contains information on HLA genotype and surface expression of HLA molecules, expression of tumour antigens, antigen processing capability, production of and response to cytokines and chemokines, apoptosis regulation and expression of adhesion/accessory molecules. Such information allows researchers to identify cells possessing specific parameters important for studies of immunity, immunogenetics, gene expression, metastasis, response to chemotherapy, and other tumour biological experimentation.

Profilo DNA

STR-PCR Data:

Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 9,10
D16S539: 9,13
D5S818: 12,13
D7S820: 8,11
THO1: 9.3,9.3
TPOX: 8
vWA: 15,18

Terreno di coltura

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) seed new cultures at 2-4 x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Saturation density approx 8 x10,000 cells/cm²

Altre note

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