A375 Cell Line human

88113005, human skin, Epithelial

• Sinonimo/i: A-375 Cells, A375-MEL Cells
• Codice UNSPSC: 41106514

Proprietà

• product name: A375 Cell Line human, 88113005
• Origine biologica: human skin
• Descrizione: Human malignant melanoma
• Modalità di accrescimento: Adherent
• Cariotipo: Modal no. = 62 (hypotriploid) 9 marker chromosomes. Normal N2, N6 and N22 present.
• Morfologia: Epithelial
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Human malignant melanoma

Descrizione della linea cellulare

Derived from a 54 year old female with malignant melanoma. The cell line produces rapidly growing amelanotic melanomas in anti-thymocyte serum treated NIH Swiss mice.

Applicazioni

A375 cell line has been used to obtain paracrine factors for prolonged culture of mesenchymal stromal cells (MSCs). It has also been used to study oncolytic activity of the peptide LTX-315.

Tumourigenicity studies

Terreno di coltura

DMEM + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:3 to 1:8 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C.

Altre note

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