OV7

96020764

• Sinonimo/i: OV-7 Cells
• Codice UNSPSC: 41106514

Proprietà

• Origine biologica: human ovary
• Modalità di accrescimento: Adherent
• Cariotipo: Not specified
• Morfologia: Epithelial
• Prodotti: VEGF
• Recettori: VEGF receptors flt and KDR
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Human ovarian cancer, stage III

Descrizione della linea cellulare

The OV7 cell line was established from solid ovarian tumour tissue taken from a 78-year-old female patient. The tumour was characterised as stage III, mixed tumour type and poorly differentiated. Immunocytochemical staining of the cells was reported to show strong staining for cytokeratin (CK), no staining for carcinoembryonic antigen (CEA) and strong staining for human milk fat globulin (HMFG-2). Detection of mRNA expression of vascular endothelial growth factor (VEGF) and its receptors flt and KDR has been reported. The patient had received treatment with chlorambucil.

Terreno di coltura

DMEM:HAMS F12 (1:1) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS) + 0.5 ug/ml hydrocortisone + 10ug/ml insulin

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Altre note

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