NMuMG

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• Codice UNSPSC: 41106514

Proprietà

• Origine biologica: mouse breast
• Confezionamento: tube of 5 μg 94081121-DNA-5UG; pkg of vial of cells 94081121-1VL
• Modalità di accrescimento: Adherent
• Cariotipo: Not specified
• Morfologia: Epithelial
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Mouse mammary gland, normal epithelial

Descrizione della linea cellulare

Initiated from the mammary gland tissue of an adult NAMRU mouse and one of a series of epithelial cell lines derived from normal glandular tissue of mice. The cells exhibit many ultrastructural features of secretory epithelium as well as desmosomes or intermediate junctions, microvilli and tonofibrils. Hemicytes or domes develop when dense monolayers are maintained for several weeks without sub-cultivation. The cells produce benign cystadenomas when inoculated into isogenic mice and are negative for type C oncornaviruses.

Applicazioni

NMuMG has been used to compare the actions of insulin like growth factor binding protein 5 (IGFBP-5) with transforming growth factor β 1 (TGFβ1) on epithelial cells. It has also been used to measure the influence of phorbol 12-myristate 13-acetate (PMA) and estradiol on bone morphogenetic protein receptor type 2 (BMPR2) gene expression.

Terreno di coltura

DMEM + 2mM Glutamine + 10ug/ml Insulin + 10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Altre note

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