BAE-1

88031149, bovine cardiovascular , Endothelial

• Codice UNSPSC: 41106514

Proprietà

• product name: BAE-1, 88031149
• Origine biologica: bovine cardiovascular
• Modalità di accrescimento: Adherent
• Cariotipo: 59 or 60
• Morfologia: Endothelial
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Bovine aortic endothelium

Descrizione della linea cellulare

Derived from the aorta of a Friesian cow. Cells form "sprouts" after confluence. Infected with Bovine Viral Diarrhoea Virus (BVDV) which is a common contaminant of ruminants and is non-hazardous to humans. Secretes proteoglycans and collagen.

Terreno di coltura

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C. There is a tendancy for the cells to grow fibroblast-like until they are confluent. Once confluent the endothelial morphology prevail

Altre note

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