JEG3

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 92120308, human placenta, Epithelial

• Codice UNSPSC: 41106514

Proprietà

• Origine biologica: human placenta
• Confezionamento: tube of 5 μg 92120308-DNA-5UG; pkg of vial of cells 92120308-1VL
• Modalità di accrescimento: Adherent
• Cariotipo: modal no. 71
• Morfologia: Epithelial
• Prodotti: Human chorionic gonadotrophin (hCG), somatomammotrophin
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Human choriocarcinoma

Descrizione della linea cellulare

One of 6 clones derived from cells implanted into a hamster cheek pouch and then propagated on irradiated feeder layers of human fibroblasts. Cell line releases chorionic gonadotrophin and somatomammotrophin and progesterone. It is also able to transform steroid precursors to oestrone and oestradiol. JEG3 has the same DNA profile as BeWo (Sigma Catalogue number 86082803); it was established by serial cloning of BeWo .

Terreno di coltura

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin, 5% CO₂; 37°C. Vacuolisation will occur at confluency.

Altre note

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