ND15

92090907

• Codice UNSPSC: 41106514

Proprietà

• Origine biologica: Mouse x Rat Hybridoma nerve
• Modalità di accrescimento: Adherent
• Cariotipo: Not specified
• Morfologia: Neuronal
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Mouse neuroblastoma x Rat neurone hybrid

Descrizione della linea cellulare

The hybrid cell line ND15 was derived after fusion of primary neonatal-rat dorsal-root-ganglion (DRG) neurons with N18Tg2, a mouse neuroblastoma (C1300)- derived azaguanine-resistant line. HAT-sensitive hybrids were selected and cloned by limiting dilution. The cells express all isoforms of N-CAM but no embryonic form. Thy 1.1 marker, P31 and PrPc can be detected as well as a weaker expression of F3 and Thy-3. When differentiated in serum free media an increased level of Thy-2 can be detected.

Applicazioni

Study of neuronal differentiation, cell adhesion and expression of neuronal proteins

Terreno di coltura

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm² by either aspirating or gently tapping the cells loose; 5% CO₂; 37°C. Cells are lightly attached therefore use of trypsin is unnecessary.

Altre note

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