CFPAC-1

91112501, human pancreas (liver metastasis), Epithelial

• Codice UNSPSC: 41106514

Proprietà

• product name: CFPAC-1, 91112501
• Origine biologica: human pancreas (liver metastasis)
• Modalità di accrescimento: Adherent
• Cariotipo: Hyperdiploid, (65-75 chromosomes)
• Morfologia: Epithelial
• Prodotti: Not specified
• Recettori: Cystic fibrosis trans-membrane regulator (CFTR)
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: metastasis
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Human Caucasian pancreatic adenocarcinoma

Descrizione della linea cellulare

CFPAC-1 is a ductal pancreatic adenocarcinoma derived by differential trypsinisation of explant cultures from a metastatic lesion in the liver of a 26 year old caucasian male with cystic fibrosis (CF). The cell line expresses cystic fibrosis trans-membrane regulator (CFTR) and ion transport properties consistent with the CF defect. CFPAC-1 cells show an epithelial morphology and polarisation with apical microvilli. Expression of cytokeratin and oncofoetal antigens characteristic of pancreatic duct cells have been described. The cells express the CF gene and show the most common CF mutation, deletion of three nucleotides result in a phenylalanine-508 deletion. CFPAC-1 cells are also tumorigenic in nude mice.

Applicazioni

Cystic fibrosis studies, genetic analysis

Terreno di coltura

IDMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4 x 10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Altre note

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