MIA-Pa-Ca-2

85062806, human pancreas, Epithelial

• Sinonimo/i: MIA PaCa-2 Cells, MIA-PaCa2 Cells, MIAPACA-2 Cells, PaCa2 Cells
• Codice UNSPSC: 41106514

Proprietà

• Nome del prodotto: MIA-Pa-Ca-2, 85062806
• Origine biologica: human pancreas
• Modalità di accrescimento: Adherent
• Cariotipo: Modal no. 61; hypotriploid
• Morfologia: Epithelial
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Human Caucasian pancreatic carcinoma

Descrizione della linea cellulare

An established cell line from an undifferentiated human pancreatic carcinoma. The tumour was taken from a 65 year old Caucasian male. The cells are large with abundant cytoplasm, exhibit a high degree of aneuploidy, have a tendency to grow on top of other cells, eventually growing free in suspension. Sensitive to L-Asparaginase.

Applicazioni

MIA-Pa-Ca-2 has been used for the assessment of thyroid receptor transcriptional activity and for α-enolase immunostaining.

Profilo DNA

STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 12,13
D16S539: 10,13
D5S818: 12,13
D7S820: 12,13
THO1: 9,10
TPOX: 9
vWA: 15

Terreno di coltura

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Altre note

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.