G-361

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 88030401, human skin, Epithelial

• Codice UNSPSC: 41106514

Proprietà

• Origine biologica: human skin
• Confezionamento: tube of 5 μg 88030401-DNA-5UG; pkg of vial of cells 88030401-1VL
• Modalità di accrescimento: Adherent
• Cariotipo: Triploid, modal n.o 69
• Morfologia: Epithelial
• Prodotti: Melanin
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Human Caucasian malignant melanoma

Descrizione della linea cellulare

Established from a malignant melanoma of a 31 year old male Caucasian. The cells produce melanin for up to 50 population doublings. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.

Applicazioni

Hormone studies

Profilo DNA

STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 11
D16S539: 15
D5S818: 11
D7S820: 8,11
THO1: 7,9
TPOX: 8,10
vWA: 15,17

Terreno di coltura

McCoy′s 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Cells are slow in attaching.

Altre note

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