FM3Ats C1.T85

87111904, mouse breast, Lymphoblast

• Codice UNSPSC: 41106514

Proprietà

• product name: FM3Ats C1.T85, 87111904
• Origine biologica: mouse breast
• Modalità di accrescimento: Suspension
• Cariotipo: Not specified
• Morfologia: Lymphoblast
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Mouse C3H mammary carcinoma

Descrizione della linea cellulare

A temperature sensitive mutant isolated from the mouse mammary cell carcinoma cell line, FM3A (Sigma Catalogue number. 87100804). Cells will not divide at 39°C (non-permissive temperature) and will grow at 33°C (permissive temperature). Electron microscope findings have shown that chromatin abnormally condenses into fragmented and compact forms in 80% of the cells at 39°C for 16 hours. This abnormal condensation does not occur at 33°C or in the FM3A cell line at either temperature.

Applicazioni

Tumourigenicity studies, chromosome condensation studies.

Terreno di coltura

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Maintain cultures between 1-3x100,000 cells/ml; 5% CO₂; 33°C.

Altre note

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