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Key Documents

5006

Sigma-Aldrich

PureCol™

Collagen powder from Bovine hide, 97% Type I with the remainder of Type III collagen, suitable for cell culture

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About This Item

Numero CE:
232-697-4
Codice UNSPSC:
12352202
NACRES:
NA.75

Livello qualitativo

100

Sterilità

sterile-filtered

Saggio

99.9%

Forma fisica

lyophilized

Confezionamento

pkg of 15 mg

tecniche

cell culture | mammalian: suitable

Specificità del legame

Peptide Source: Elastin

Peptide Source: Fibrinogen

Condizioni di spedizione

wet ice

Temperatura di conservazione

−20°C

Categorie correlate

Descrizione generale

PureCol™ collagen is approximately 97% Type I with the remainder being comprised of Type III collagen. It is soluble atelo-collagen. It contains high monomer content as measured by gel permeation chromatography.

It is ideal for coating of surfaces and providing preparations of thin layers for culturing cells. PureCol™, lyophilized form is not recommended for the formation of a solid gel. PureCol™ collagen is provided in user-friendly packaging for use and storage. This product is supplied as a sterile lyophilized powder.

Applicazioni

PureCol™ has been used:
  • in collagen gel preparation for the 3D sprouting assay with human umbilical vein endothelial cells (HUVEC) spheroids 
  • to coat Costar® Transwell® Inserts plates before cell migration assays with bladder smooth muscle cells (BMSC)
  • to coat culture plates for collagen degradation assay with breast cancer cell lines

Nota sulla preparazione

This product is supplied as a lyophilized powder with 15 mg of bovine collagen. When reconstituted with 5 ml of sterile 0.01 N HCl, a concentration of approximately 3 mg/ml is achieved.

Note legali

Costar is a registered trademark of Corning, Inc.
PureCol is a trademark of Advanced BioMatrix, Inc.
Transwell is a registered trademark of Corning, Inc.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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