HOS

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 87070202, human bone, Epithelial-like

• Codice UNSPSC: 41106514

Proprietà

• Origine biologica: human bone
• Confezionamento: tube of 5 μg 87070202-DNA-5UG; pkg of vial of cells 87070202-1VL
• Modalità di accrescimento: Adherent
• Cariotipo: 2n = 46, hyperdiploid
• Morfologia: Epithelial-like
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Human Caucasian osteosarcoma, TE85

Descrizione della linea cellulare

Derived from a 13 year old female caucasian. Cells are sensitive to both virus and chemical transformation and exhibit a flat morphology, low saturation density and low plating efficiency in soft agar. HOS and 143B (Sigma Catalogue number 91112502) originated from the same host: 143B is TK- and originated indirectly from HOS which is TK+ (Skovgaard et al 2010 PMID: 20544518).

Applicazioni

Virus, transformation and biochemical studies

Profilo DNA

STR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 12
D16S539: 10,13
D5S818: 13
D7S820: 11,12
THO1: 6
TPOX: 8,11
vWA: 18

Terreno di coltura

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Altre note

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