U-2 OS Cell Line human

92022711, human bone, Not specified

• Sinonimo/i: U-2OS Cells, U2-OS Cells, U20S Cells, U2OS Cells
• Codice UNSPSC: 41106514

Proprietà

• product name: U-2 OS Cell Line human, osteosarcoma, 92022711
• Origine biologica: human bone
• Modalità di accrescimento: Adherent
• Cariotipo: Not specified
• Morfologia: Not specified
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Human Osteosarcoma

Descrizione della linea cellulare

Cell line derived in 1964 from a moderately differentiated sarcoma of the tibia of a 15 year old girl.

Applicazioni

U-2 OS has been used to study:

• the importance of cyclin D1 for the activity of lithocholic acid hydroxyamide (LCAHA)
• the interaction of human single-stranded DNA binding protein 1 (hSSB1) with bloom syndrome protein helicase (BLM helicase)
• calcium-mediated actin reset (CaAR) in response to physiological changes

Profilo DNA

STR-PCR Data: Amelogenin: X
CSF1PO: 13
D13S317: 13
D16S539: 11,12
D5S818: 11
D7S820: 11,12
THO1: 6,9.3
TPOX: 11,12
vWA: 14,18

Terreno di coltura

McCoy′s 5a medium with 1.5 mM Glutamine; 10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split subconfluent 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh medium, aspirate and dispense into new culture flasks.

Altre note

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