A673

85111504, human muscle, Epithelial-like

• Codice UNSPSC: 41106514

Proprietà

• Nome del prodotto: A673, 85111504
• Origine biologica: human muscle
• Confezionamento: tube of 5 μg 85111504-DNA-5UG; pkg of vial of cells 85111504-1VL
• Modalità di accrescimento: Adherent
• Cariotipo: Near Diploid
• Morfologia: Epithelial-like
• Prodotti: Transforming growth factor (Tramnsforming Growth Factor (TGF)) - ß
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Human Ewing sarcoma

Descrizione della linea cellulare

Derived from a 15 year old female patient. The cells form colonies on soft agar and tumours in antithymocyte serum treated mice.

The cell line A673 was originally described in 1973 as derived from a rhabdomyosarcoma, however, more recent analysis of the cells using advanced diagnostic techniques determined the cells to have characteristics of Ewing sarcoma rather than rhabdomyosarcoma. See citations for more information about this tumour status.

Applicazioni

Tumourigenicity studies

Profilo DNA

STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 8,13
D16S539: 11
D5S818: 11,12
D7S820: 10,12
THO1: 9.3
TPOX: 8
vWA: 15,18

Terreno di coltura

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:3 to 1:10, i.e., seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37 °C.

Altre note

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