BRIN-BG5

10033001, rat pancreas (islets), Epitheloid

• Sinonimo/i: BRIN
• Codice UNSPSC: 41106514

Proprietà

• Nome del prodotto: BRIN-BG5, 10033001
• Origine biologica: rat pancreas (islets)
• Modalità di accrescimento: Adherent
• Cariotipo: Modal chromosome number 66 up to passage 50
• Morfologia: Epitheloid
• Prodotti: BRIN-BG5 is a stable, glucose-responsive insulin secreting beta cell line expressing insulin, glucokinase and IAPP as detected by immunocytochemistry. BRIN-BG5 cells express the GLUT2 glucose transporter.
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Condizioni di spedizione: dry ice

Descrizione

Origine della linea cellulare

A rat pancreatic Beta cell: RINm5F hybrid cell line. Insulin-secreting cell line

Descrizione della linea cellulare

BRIN-BG5 is a hybrid cell line formed by the electrofusion of a primary culture of NEDH rat pancreatic islets with RINm5F (a cell line derived from a NEDH rat insuliinoma). BRIN-BG5 has been shown to be tumourigenic when transplanted into a SCID mouse host. BRIN-BG5 has been mycoplasma eradicated at ECACC and the stocks available for supply have undergone a further 10 passages without detection of mycoplasma.

Terreno di coltura

RPMI 1640 (R0883) + 2 mM L-Glutamine (G7513) + FBS / FCS (F2442)

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:8 to 1:10, i.e. seeding at 2-4 x 10⁴ cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37 °C. Population doubling approximately28hrs. At confluence 105 cells/cm² can be expected.

Altre note

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Esclusione di responsabilità

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.