BF-2

21713, fish caudal trunk, Fibroblast-like

• Codice UNSPSC: 41106514

Proprietà

• product name: BF-2, 00021713
• Origine biologica: fish caudal trunk
• Modalità di accrescimento: Adherent
• Cariotipo: Not specified
• Morfologia: Fibroblast-like
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Fish bluegill fry

Descrizione della linea cellulare

The cell line BF-2 has been derived from a trypsinised suspension of pooled caudal portions of the trunk of 1 year old fingerlings (Bluegill fry, Lepomis macrochirus). Cells have been reported to be susceptible to a variety of fish viruses including viral haemorrhagic septicaemia virus (VHSV), infectious hematopoietic necrosis virus (IHNV), spring viraemia of carp virus (SVCV) and pike fry rhabdovirus (PFRV).

Applicazioni

Fish virus studies

Terreno di coltura

EMEM(HBSS) (M5775) or EMEM (EBSS) (M2279) + 2mM L-Glutamine (G7513) + 850 mg/L Sodium Bicarbonate(NaHCO₃) (S8761) + 1% Non Essential Amino Acids(NEAA) (M7145) + 120 mg/l Sodium Pyruvate(NaP) (S8636) + 10% FBS / FCS (F2442); a mixture of MEM (HBSS) and MEM (EBSS) with 1-2% FBS is best for stability of monolayer and maintenance medium

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:4 to 1:6 using 0.25% trypsin or trypsin/EDTA; cells replicate at 15 - 33°C, optimal temperature is 23 - 26°C; monolayer is stable for at least 10 days in maintenance medium (at 15 or 16°C for about 21 days). Fish c

Altre note

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