RTG-2

90102529, fish ovary, Fibroblast

• Codice UNSPSC: 41106514

Proprietà

• product name: RTG-2, 90102529
• Origine biologica: fish ovary
• Modalità di accrescimento: Adherent
• Cariotipo: 2n = 60
• Morfologia: Fibroblast
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Trout Rainbow gonad tissue

Descrizione della linea cellulare

From pooled male/female gonad tissue of yearling rainbow trout (Oncorhynchus mykiss). First cell line to be established from cold-blooded vertebrates. 8-fold difference in growth rate in temperature range 4-24°C. Susceptible to infectious pancreatic necrosis virus, Egtved virus and arboviruses (EEE, VEE).

Applicazioni

Virus studies: Inf. pacr. necrotic virus, Egtved virus, arboviruses (EEE, VEE)

Terreno di coltura

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Passage using 0.25% trypsin below 24°C. Cells may be seeded as few as 3x10,000 cells/ml 5% CO₂; 24°C. Fish cell lines detach easily during transit if the culture is very youn

Altre note

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