CA200
cAMP Enzyme Immunoassay Kit, Direct
Sinonimo/i:
cAMP detection kit
Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
About This Item
Codice UNSPSC:
12161503
NACRES:
NA.84
Prodotti consigliati
tecniche
ELISA: suitable
Livello qualitativo
Condizioni di spedizione
wet ice
Temperatura di conservazione
−20°C
Descrizione generale
Adenosine 3′,5′-cyclic monophosphate (cAMP) is a second messenger in many physiological processes. It is synthesized from adenosine triphosphate (ATP) in the presence of enzyme adenylate cyclase.
Non-radioactive, competitive immunoassay for the quantitation of total cAMP in tissue and cell cultures treated with .1M HCL.The kit cAMP uses a cAMP polyclonal antibody to competitively bind the cAMP in the sample or alkaline phosphatase moleule that has cAMP covalently attachd to it.
Applicazioni
Please refer to the attached Protocolfor details.
cAMP Enzyme Immunoassay Kit, Direct has been used to measure cAMP levels in cell culture medium virulent and attenuated macrophages. It has also been used to quantify cAMP synthesis in olfactory epithelium.
Azioni biochim/fisiol
Adenosine 3′,5′-cyclic monophosphate (cAMP) is shown to be involved in the cardiovascular and nervous systems, immune mechanisms, cell growth and differentiation, and general metabolism. Deciphering cAMP modulation in regulatory pathways may help in understanding of the physiology and pathology of many disease states.
Altre note
Allow reagents to warm at room temperature for 30 minutes prior to opening.
Solo come componenti del kit
N° Catalogo
Descrizione
- 0.1 M Hydrochloric Acid 1 x 30
- Acetic Anhydride 1 x 1
- cAMP-Alkaline Phosphatase Conjugate 1 x 5
- cAMP EIA Antibody Rabbit Anti-cAMP 1 x 5
- 5 Cycle Logit-Log Paper 1 x 1
- Cyclic AMP Standard 1 x 0.5
- Direct cAMP Assay Layout Sheet 1 x 1
- Goat Anti-Rabbit IgG Coated 96 Well Microtiter Plate 1 ea
- Neutralizing Reagent 1 x 6
- p-Nitrophenyl Phosphate Substrate Solution 1 x 20
- Plate Sealer 1 ea
- Stop Solution 1 x 5
- Triethylamine 1 x 2
- Wash Buffer Concentrate 1 x 30
Vedi tutto (14)
Avvertenze
Danger
Indicazioni di pericolo
Classi di pericolo
Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1A - STOT SE 3
Organi bersaglio
Respiratory system
Rischi supp
Codice della classe di stoccaggio
3 - Flammable liquids
Punto d’infiammabilità (°F)
120.2 °F
Punto d’infiammabilità (°C)
49 °C
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