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C6788

Sigma-Aldrich

4-Chloro-1-naphthol

peroxidase substrate, tablet, suitable for use in immunoblotting procedures

Sinonimo/i:

4-CN, 4-chloronaphthalen-1-ol

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About This Item

Formula condensata:
ClC10H6OH
Numero CAS:
604-44-4
Peso molecolare:
178.61
Beilstein:
509750
Numero CE:
210-068-5
Numero MDL:
MFCD00003974
Codice UNSPSC:
12352204
ID PubChem:
57654035
NACRES:
NA.83

Nome del prodotto

4-Chloro-1-naphthol, tablet

Stato

tablet

Livello qualitativo

200

Punto di fusione

118-121 °C (lit.)

Solubilità

methanol: 10 mL, clear, colorless

Temperatura di conservazione

−20°C

Stringa SMILE

Oc1ccc(Cl)c2ccccc12

InChI

1S/C10H7ClO/c11-9-5-6-10(12)8-4-2-1-3-7(8)9/h1-6,12H
LVSPDZAGCBEQAV-UHFFFAOYSA-N

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Specificità

4-Chloro-1-Naphthol is a peroxidase substrate suitable for use in immunoblotting procedures. This substrate produces an insoluble end product that is blue in color and can be observed visually.

Applicazioni

4-Chloro-1-naphthol has been used as a substrate in enzyme-linked immunospot (ELISpot) assay for detection of the spots. It has also been used as a precipitating chromogenic substrate for visualizing the antigen−antibody-reactive bands following western blotting.

Quantità

Contains 30 mg substrate per tablet.

Ricostituzione

Dissolve 1 tablet in 10 ml of methanol. Add 2 ml of methanol stock solution to 10 ml of triethanolamine buffered saline, pH 7.5. Add 5 μl of fresh 30% hydrogen peroxide (Sigma Product No. H-1009) immediately prior to use.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

dust mask type N95 (US), Eyeshields, Gloves

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Certificati d'analisi (COA)

Lot/Batch Number
SLBX4735
SLBN4295V
SLBG6510V
SLBC8821V
120M8203V

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Parasitology Research, 99(4), 398-404 (2006)
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Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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