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Key Documents

C4249

Sigma-Aldrich

Cystatin B human

recombinant, expressed in E. coli, solid, ≥95% (SDS-PAGE)

Sinonimo/i:

CPI-B, CST6, CSTB, EPM1, Liver Thiol Proteinase Inhibitor, PME, STFB, Stefin B

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About This Item

Codice UNSPSC:
12352202
NACRES:
NA.77

product name

Cystatin B human, recombinant, expressed in E. coli

Ricombinante

expressed in E. coli

Livello qualitativo

200

Descrizione

contains a N-terminal 7X His-tag

Saggio

≥95% (SDS-PAGE)

Forma fisica

solid

PM

apparent mol wt ~13 kDa by SDS-PAGE (reducing)

N° accesso NCBI

NP_000091

Temperatura di conservazione

−20°C

Informazioni sul gene

human ... CSTB(1476)

Descrizione generale

Cystatin B is a member of the type 1 (stefins) superfamily of cystatins. Cystatin B is encoded by the CSTB gene and functions as an intracellular thiol protease inhibitor regulating the activity of members of the papain family and cathepsins B, H, and L. Cystatin B may also protect cellular components from the effects of proteases that leak from lysosomes. Mutations in the CSTB gene are thought to be the primary defect in patients with Progressive Myoclonus Epilepsy (EPM1).

Applicazioni

Suitable for use as a cysteine-protease inhibitor. Is tested for suitability as an inhibitor of the enzymatic activity of papain.

Azioni biochim/fisiol

Cystatin B was shown to interact with the protein RACK-1, β-spectrin and neurofilament light chain NF-L forming a unique multiprotein complex specifically in the cerebellum, confirming its function in the brain.

Ricostituzione

Cystatin B should be reconstituted at 100 μg/mL in steile 25 mM Tris pH 7.5 and 100 mM NaCl. Reconstituted product should be stored in single-use aliquots at -70° C and should be stable for at least 3 months.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

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Rossella Di Giaimo et al.
Human molecular genetics, 11(23), 2941-2950 (2002-10-24)
Cystatin B is an anti-proteolytic polypeptide implicated in progressive myoclonus epilepsy (EPM1), a degenerative disease of the central nervous system. The knock-out mouse model of the disease shows apoptosis of the cerebellar granule cells. We have identified five recombinant proteins
Hana Dvořáková et al.
Frontiers in cellular and infection microbiology, 10, 66-66 (2020-03-17)
Schistosomula (the post-infective stages) of the neurotropic schistosome Trichobilharzia regenti possess multiple isoforms of cathepsin B1 peptidase (TrCB1.1-TrCB1.6) with involvement in nutrient digestion. The comparison of substrate preferences of TrCB1.1 and TrCB1.4 showed that TrCB1.4 had a very narrow substrate
Amanda Phuong Tran et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 38(23), 5399-5414 (2018-05-16)
Severed axon tips reform growth cones following spinal cord injury that fail to regenerate, in part, because they become embedded within an inhibitory extracellular matrix. Chondroitin sulfate proteoglycans (CSPGs) are the major axon inhibitory matrix component that is increased within
Vincenzo Lionetti et al.
PloS one, 10(7), e0133810-e0133810 (2015-07-24)
Pectin is secreted in a highly methylesterified form and partially de-methylesterified in the cell wall by pectin methylesterases (PMEs). PME activity is expressed during plant growth, development and stress responses. PME activity is controlled at the post-transcriptional level by proteins

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