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Key Documents

C-39425

Sigma-Aldrich

Preadipocyte Growth Medium

SupplementMix containing all media supplements pre-mixed in one vial, 1 Mix for 500 ml

Sinonimo/i:

Recommended with C-27411

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About This Item

Codice UNSPSC:
12352207
NACRES:
NA.71

Forma fisica

aqueous solution

Confezionamento

pkg of 1 vial

tecniche

cell culture | mammalian: suitable

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

Descrizione generale

Preadipocyte Growth Medium SupplementMix (basal medium sold separately)

To form a complete medium, it should be combined with the basal medium: C-27411

Applicazioni

Preadipocyte Growth Medium is a low-serum (5% V/V) medium developed for the undifferentiated expansion of human preadipocytes. The media are optimized for primary human cells, but can also be used for murine preadipocytes.Recommended for:Human White Preadipocytes (HWP)

Componenti

Part of a Cell Culture System offered in a variety of formats:Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMixGrowth Medium Kit: Includes Basal Medium and SupplementPackSupplementMix: Contains all media supplements premixed in one vialSupplementPack: Contains all media supplements as individual vials

Qualità

All lots of PromoCell Preadipocyte/Adipocyte Media are subjected to comprehensive quality control tests using primary human white preadipocytes. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the tested preadipocytes. In addition, the Preadipocyte Differentiation Medium/Adipocyte Nutrition Medium are tested for the capacity to induce the terminal differentiation of preadipocytes into mature adipocytes. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Nota sulla preparazione

Final supplement concentrations after addition to the basal medium:Fetal Calf Serum: 0.05 ml / mlEndothelial Cell Growth Supplement: 0.004 ml / mlEpidermal Growth Factor (recombinant human): 10 ng / mlHydrocortisone: 1 µg / mlHeparin: 90 µg / ml

Stoccaggio e stabilità

Store the SupplementMix at -20°C in the dark immediately after arrival. If stored properly, it is stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.

Mantenimento delle subcolture

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Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Articoli

Primary Human Preadipocytes and Preadipocyte/Adipocyte Growth & Differentiation Media

Primary human white preadipocytes and culture media for the expansion of undifferentiated preadipocytes, differentiation to adipocytes, and cultivation of mature adipocytes. Protocols for handling, cell passaging, media, and product use.

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