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Key Documents

C1117

Sigma-Aldrich

Anti-Cy5 antibody, Mouse monoclonal

clone CY5-15, purified from hybridoma cell culture

Sinonimo/i:

Cy5 Antibody, Cy5 Antibody - Anti-Cy5 antibody, Mouse monoclonal, Monoclonal Anti-Cy5 antibody produced in mouse

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About This Item

Numero MDL:
MFCD08705581
Codice UNSPSC:
12352203
NACRES:
NA.41

Origine biologica

mouse

Livello qualitativo

200

Coniugato

unconjugated

Forma dell’anticorpo

purified immunoglobulin

Tipo di anticorpo

primary antibodies

Clone

CY5-15, monoclonal

Forma fisica

buffered aqueous solution

Confezionamento

antibody small pack of 25 μL

Concentrazione

~1.5 mg/mL

tecniche

direct ELISA: suitable
dot blot: 1-2 μg/mL using cell protein extracts labeled with Cy5
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
microarray: suitable

Isotipo

IgG1

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

modifica post-traduzionali bersaglio

unmodified

Descrizione generale

CyDyes are a family of fluorophores that can be used for labeling proteins, peptides, DNA, RNA, and other biomolecules. These dyes are small, pH insensitive (between pH 3 and pH 10), and soluble in aqueous solution and have tolerance to DMSO. They are more photo-stable than fluorescein, have high molar extinction coefficients, and favorable quantum yields. Due to their physical and chemical properties, they are used (mainly Cy3 and Cy5) in many different biological assays such as: DNA microarrays, protein microarrays, two-dimensional protein analysis (2D gels), fluorescence resonance energy transfer (FRET), and immunocytochemistry.
Mouse monoclonal clone CY5-15 anti-Cy5 antibody recognizes Cy5 and AlexaFluor647 labeled proteins, but not Cy3 labeled proteins.

Specificità

Monoclonal Anti-Cy5 recognizes Cy5 and AlexaFluor647 labeled proteins, but not Cy3 labeled proteins.
The antibody recognizes Cy5 and AlexaFluor 647 labeled proteins, but not Cy3 labeled proteins.

Immunogeno

mixture of proteins labeled with Cy5.

Applicazioni

Anti-Cy5 antibody, Mouse monoclonal has been used in immunofluorescence assays and western blotting.
Mouse monoclonal clone CY5-15 anti-Cy5 antibody is used to tag CyDye 5 for detection and quantitation and signal applification by or within immunocytochemical and immunohistochemical (IHC) techniques such as ELISA, immunoprecipitation, immunocytochemistry, dot blot assay, and protein microarrays. Anti-Cy5 antibody is an important tool for Cy5 signal amplification in assays where biomolecules are labeled with Cy5.

Stato fisico

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Dissecting the role of COPI complexes in influenza virus infection
Sun E, et al.
Journal of Virology, 87(5), 2673-2685 (2013)
Eileen Sun et al.
Journal of virology, 87(5), 2673-2685 (2012-12-21)
As an obligate pathogen, influenza virus requires host cell factors and compartments to mediate productive infection and to produce infectious progeny virus. Recently, several small interfering RNA (siRNA) knockdown screens revealed influenza virus host dependency proteins, all of which identified
Jiang He et al.
PLoS pathogens, 9(10), e1003701-e1003701 (2013-10-17)
As an obligatory pathogen, influenza virus co-opts host cell machinery to harbor infection and to produce progeny viruses. In order to characterize the virus-host cell interactions, several genome-wide siRNA screens and proteomic analyses have been performed recently to identify host
Masaaki Kuwajima et al.
PloS one, 15(1), e0226797-e0226797 (2020-01-16)
Analysis of neuronal compartments has revealed many state-dependent changes in geometry but establishing synapse-specific mechanisms at the nanoscale has proven elusive. We co-expressed channelrhodopsin2-GFP and mAPEX2 in a subset of hippocampal CA3 neurons and used trains of light to induce

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