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B8931

Sigma-Aldrich

Magenta-Gal

reagent for selection of recombinant bacterial clones

Sinonimo/i:

Red-Gal, 5-Bromo-6-chloro-3-indolyl β-D-galactopyranoside

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About This Item

Formula empirica (notazione di Hill):
C14H15BrClNO6
Numero CAS:
93863-88-8
Peso molecolare:
408.63
Numero MDL:
MFCD02683897
Codice UNSPSC:
12352200
ID PubChem:
329773145
NACRES:
NA.85

Grado

for molecular biology

Livello qualitativo

200

Sterilità

non-sterile

Nome Commerciale

BioReagent

Saggio

≥98.0% (HPLC)

Stato

powder

Solubilità

methanol: soluble

Compatibilità

suitable for substrate for β-galactosidase

Temperatura di conservazione

−20°C

Stringa SMILE

OC[C@H]1O[C@H](Oc2c[nH]c3cc(Cl)c(Br)cc23)[C@H](O)[C@@H](O)[C@H]1O

InChI

1S/C14H15BrClNO6/c15-6-1-5-8(2-7(6)16)17-3-9(5)22-14-13(21)12(20)11(19)10(4-18)23-14/h1-3,10-14,17-21H,4H2/t10-,11+,12+,13-,14+/m1/s1
CHRVKCMQIZYLNM-HTOAHKCRSA-N

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Categorie correlate

Descrizione generale

Red-Gal is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Red-Gal is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.
Red-Gal is a chromogenic substrate for b-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Red-Gal is designed to replace X-Gal in the blue-white selection of recombinant bacterial colonies with the lac+ phenotype.

Applicazioni

Chromogenic substrate for β-galactosidase producing a red or magenta insoluble product.
Magenta-Gal has been used for staining synthetic cytoplasmic or nuclear LacZ mRNA. It has also been used to detect the expression of the EphB1-β-gal fusion protein. ,Magenta-Gal is suitable for use in the selection of recombinant bacterial colonies with the lac+ phenotype.

Principio

When Red-Gal is hydrolyzed by β-galactosidase, the resulting product will form a red precipitate. Lac+ colonies grown in the presence of Red-Gal turn an intense red color, allowing for easy differentiation between lac+ and lac- colonies.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

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Certificati d'analisi (COA)

Lot/Batch Number
BCBK8608V
BCBJ5700V
1403239V
1403239
102K2506

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Whole-mount in situ hybridization and immunohistochemistry in Xenopus embryos
In Situ Hybridization Methods, 151-167 (2015)
George Chenaux et al.
The European journal of neuroscience, 34(10), 1620-1633 (2011-11-23)
EphB receptor tyrosine kinases direct axonal pathfinding through interactions with ephrin-B proteins following axon-cell contact. As EphB:ephrin-B binding leads to bidirectional signals, the contributions of signaling into the Eph-expressing cell (forward signaling) or the ephrin-expressing cell (reverse signaling) cannot be
Susan C McCutcheon et al.
BMC biology, 8, 89-89 (2010-06-24)
Reporter genes are widely used in biology and only a limited number are available. We present a new reporter gene for the localization of mammalian cells and transgenic tissues based on detection of the bglA (SYNbglA) gene of Caldocellum saccharolyticum
Yeon-Jin Kim et al.
Developmental biology, 397(1), 129-139 (2014-12-03)
Members of the fibroblast growth factor (FGF) family play important roles during various developmental processes including eye development. FRS (FGF receptor substrate) proteins bind to FGFR and serve as adapters for coordinated assembly of multi-protein complexes involved in Ras/MAPK and
Maria Belen Jaurena et al.
Nature communications, 6, 7476-7476 (2015-06-24)
All cranial placode progenitors arise from a common precursor field anterior to the neural plate, the pre-placodal region (PPR). We showed that transcription factor Zic1, expressed at the anterior neural plate, is necessary and sufficient to promote placode fate. Here

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