B4882
Bromelain from pineapple stem
≥3 units/mg protein
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About This Item
Numero CAS:
Numero CE:
Numero MDL:
Codice UNSPSC:
12352204
NACRES:
NA.54
Prodotti consigliati
Forma fisica
lyophilized powder
Livello qualitativo
Attività specifica
≥3 units/mg protein
Composizione
Protein, ≥30% biuret
Temperatura di conservazione
−20°C
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Applicazioni
Bromelain from pineapple stem has been used:
- to evaluate the aqueous micellar two-phase systems composed of ionic liquids as co-surfactants
- as a positive control in sodium dodecyl sulfate (SDS) / polyacrylamide gel electrophoresis (PAGE) and in cells proliferation assay
- to study its potential analgesic effect and to evaluate its hydrolyzingeffects on proenkephalin (PENK)-derived peptides containing enkephalin sequences
Bromelain may be used to inhibit the biosysnthesis of proinflammatory prostaglandins. It may be used to reduce clotting efficiency. Bromelain, from pineapple stem, has been used to make enzymatic hydrolysates of honeybee-collected pollen .
Azioni biochim/fisiol
Bromelain is a cysteine endopeptidase with broad specificity for cleavage of proteins. Bromelain may be from a stem or piece of fruit. Product B4882 is from pineapple stem. Product P3169 (pGlu-Phe-Leu-p-nitroanilide) may be used as a chromogenic substrate for the assay of bromelain.
Definizione di unità
One unit will release 1.0 micromole of p-nitrophenol from N-alpha-CBZ-L-Lysine p-nitrophenyl ester per minute at pH 4.6 at 25 °C. One old titrimetric unit (pH 4.5 at 45 °C) is equivalent to approximately 1.7 new units (pH 4.6 at 25 °C).
Nota sulla preparazione
The product may be suspended in acetate buffer, pH 4.5 at 1 mg/mL concentration, yielding a hazy, off-white suspension.
Inibitore
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Substrato
N° Catalogo
Descrizione
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Avvertenze
Danger
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Organi bersaglio
Respiratory system
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
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I clienti hanno visto anche
Jodie Scache et al.
Scientific reports, 12(1), 22129-22129 (2022-12-23)
Sialylation of cell surface glycans plays an essential role in cell-cell interaction and communication of cells with their microenvironment. Among the tools that have been developed for the study of sialylation in living cells, metabolic oligosaccharide engineering (MOE) exploits the
In vitro antiproliferative activity of fresh pineapple juices on ovarian and colon cancer cell lines
Gani MBA, et al.
International Journal of Peptide Research and Therapeutics, 21(3), 353-364 (2015)
Recovery of bromelain from pineapple stem residues using aqueous micellar two-phase systems with ionic liquids as co-surfactants
Vicente FA, et al.
Process. Biochem., 51(4), 528-534 (2016)
C B Lourenço et al.
International journal of cosmetic science, 38(5), 535-540 (2016-02-03)
Bromelain is a mixture of proteolytic enzymes found in various tissues of the pineapple plant (Ananas comosus) and other species of Bromeliaceae. Owing to its proteolytic activity, bromelain has been used in the food, medical, pharmaceutical and cosmetic industries, for
Marco Esti et al.
Journal of agricultural and food chemistry, 59(7), 3391-3397 (2011-03-09)
The influence of potential inhibitors, naturally present in wine, on the activity of stem bromelain was investigated in order to evaluate the applicability of this enzyme for protein stabilization in white wine. Bromelain proteolytic activity was tested against a synthetic
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