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B4500

Sigma-Aldrich

Nα-Benzoyl-L-arginine ethyl ester hydrochloride

trypsin substrate, chromogenic, ≥97% (HPLC), powder

Sinonimo/i:

BAEE

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About This Item

Formula empirica (notazione di Hill):
C15H22N4O3 · HCl
Numero CAS:
2645-08-1
Peso molecolare:
342.82
Beilstein:
3781694
Numero CE:
220-157-0
Numero MDL:
MFCD00012579
Codice UNSPSC:
12352204
ID PubChem:
24891777
NACRES:
NA.32

product name

Nα-Benzoyl-L-arginine ethyl ester hydrochloride, trypsin substrate

Livello qualitativo

200

Saggio

≥97% (HPLC)

Forma fisica

powder

Solubilità

water: 50 mg/mL, clear, colorless

Temperatura di conservazione

2-8°C

Stringa SMILE

Cl[H].CCOC(=O)[C@H](CCCNC(N)=N)NC(=O)c1ccccc1

InChI

1S/C15H22N4O3.ClH/c1-2-22-14(21)12(9-6-10-18-15(16)17)19-13(20)11-7-4-3-5-8-11;/h3-5,7-8,12H,2,6,9-10H2,1H3,(H,19,20)(H4,16,17,18);1H/t12-;/m0./s1
HIXDELXKSSLIKB-YDALLXLXSA-N

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Descrizione generale

Nα-Benzoyl-L-arginine ethyl ester hydrochloride is a substrate for trypsin. It is a nonprotein substrate used in spectroscopic assays.

Applicazioni

  • N

  • α-Benzoyl-L-arginine ethyl ester hydrochloride has been used as a substrate:
  • in peptidyl arginine deiminase (PPAD) assay in P. gingivalis vesicles
  • for assaying proteolytic activity of enzymatic extracts of papaya plant parts
  • in trypsin activity assay of the tail tendon fascicles from rat

Substrati

The prototype substrate for trypsin.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

Certificati d'analisi (COA)

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Maria T Dulay et al.
Analytical chemistry, 77(14), 4604-4610 (2005-07-15)
Trypsin is covalently linked to a photopolymerized sol-gel monolith modified by incorporating poly(ethylene glycol) (PSG-PEG) for on-column digestion of N(alpha)-benzoyl-l-arginine ethyl ester (BAEE) and two peptides, neurotensin and insulin chain B. The coupling of the enzyme to the monolith is
The mechanical, structural, and compositional changes of tendon exposed to elastase
Grant TM, et al.
Annals of Biomedical Engineering, 43(10), 2477-2486 (2015)
Weijun Wang et al.
Scientific reports, 9(1), 13630-13630 (2019-09-22)
Cellulases play important roles in the dietary fibre digestion in pigs, and have multiple industrial applications. The porcine intestinal microbiota display a unique feature in rapid cellulose digestion. Herein, we have expressed a cellulase gene, p4818Cel5_2A, which singly encoded a
Reynaldo Villalonga et al.
Biotechnology and bioengineering, 81(6), 732-737 (2003-01-17)
Bovine pancreatic trypsin was modified by the mono-6-amino-6-deoxy derivatives of alpha-, beta-, and gamma-cyclodextrin through a transglutaminase-catalyzed reaction. The trypsin-cyclodextrin conjugates, containing about 3 mol of oligosaccharide per mole of protein, were tested for their catalytic and stability properties. The
Mohammad K Hossain et al.
Colloids and surfaces. B, Biointerfaces, 181, 85-93 (2019-05-28)
Electric current responsive magnetic composite particles are prepared in three steps. In the first step, spherical and mesoporous submicrometer-sized magnetic iron oxide (Fe3O4) core particles are prepared by solvothermal method. Then magnetic Fe3O4 particles are functionalized with amine groups using
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