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Key Documents

B3275

Sigma-Aldrich

Monoclonal Anti-Rabbit Immunoglobulins–Biotin antibody produced in mouse

clone RG-16, purified immunoglobulin, buffered aqueous solution

Sinonimo/i:

Monoclonal Anti-Rabbit Immunoglobulins (IgG, IgA, IgM)

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About This Item

Codice UNSPSC:
12352203
NACRES:
NA.46

Origine biologica

mouse

Livello qualitativo

200

Coniugato

biotin conjugate

Forma dell’anticorpo

purified immunoglobulin

Tipo di anticorpo

secondary antibodies

Clone

RG-16, monoclonal

Forma fisica

buffered aqueous solution

Reattività contro le specie

rabbit

Non deve reagire con

human
pig, chicken, bovine, horse, human, guinea pig, turkey, rat, canine, goat, feline, sheep

tecniche

direct ELISA: 1:60,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,500
western blot: 1:200,000-1:400,000 using using an assay detecting actin in total cell extract of HeLa cells (5-10 μg per well)
western blot: 1:200,000

Isotipo

IgG1

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

modifica post-traduzionali bersaglio

unmodified

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Categorie correlate

Descrizione generale

Reacts with an epitope on the heavy chain of rabbit IgG, IgA and IgM, which is sensitive to reduction. No cross-reactivity with human IgG, IgA and IgM.

Specificità

Binds only rabbit Igs. Does not react with reduced rabbit Igs.

Applicazioni

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
The mouse monoclonal anti-Rabbit IgG (heavy chain-specific) peroxidase conjugated antibody can be used as a secondary antibody for western blot analysis of protein preps from E. coli cultures at a concentration of 1:10000 diluted in PBSt for 1 hour at room temperature.

Azioni biochim/fisiol

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. IgA antibody is secretory antibody and is present abundantly in mucous linings of gastrointestinal, respiratory and genitourinary tracts, tears and saliva. IgM antibodies are present as pentamers in the serum and are produced in response to antigens.

Stato fisico

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

nwg

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Takeshi Hayashi et al.
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism, 23(10), 1117-1128 (2003-10-04)
The endoplasmic reticulum (ER), which plays important roles in apoptosis, is susceptible to oxidative stress. Because reactive oxygen species (ROS) are robustly produced in the ischemic brain, ER damage by ROS may be implicated in ischemic neuronal cell death. We
Milena Penkowa et al.
Journal of neuroscience research, 73(4), 481-496 (2003-08-05)
6-aminonicotinamide (6-AN) is a niacin antagonist, which leads to degeneration of gray matter astrocytes mainly in the brainstem. We have examined the role of interleukin-6 (IL-6) in this degenerative process by using transgenic mice with astrocyte-targeted IL-6 expression (GFAP-IL6 mice).
Takeshi Hayashi et al.
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism, 23(8), 949-961 (2003-08-07)
Although the endoplasmic reticulum (ER) is implicated in neuronal degeneration in some situations, its role in delayed neuronal cell death (DND) after ischemia remains uncertain. The authors speculated that ER stress is involved in DND, that it is reduced by
M Penkowa et al.
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism, 20(8), 1174-1189 (2000-08-19)
To study the importance of metallothionein-I and -II (MT-I+II) for brain inflammation and regeneration, the authors examined normal and MT-I+II knock-out (MT-KO) mice subjected to a cortical freeze injury. Normal mice showed profound neurodegeneration, inflammation, and gliosis around the injury
Albert Quintana et al.
Journal of neuroscience research, 82(5), 701-716 (2005-11-04)
Tumor necrosis factor-alpha (TNF-alpha) is one of the mediators dramatically increased after traumatic brain injury that leads to the activation, proliferation, and hypertrophy of mononuclear, phagocytic cells and gliosis. Eventually, TNF-alpha can induce both apoptosis and necrosis via intracellular signaling.
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