APOAC
Annexin V-Cy3™ Apoptosis Detection Kit
Sinonimo/i:
Red fluorescent Annexin V apoptosis assay
Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
About This Item
Codice UNSPSC:
12352207
NACRES:
NA.32
Prodotti consigliati
impiego
sufficient for 200 tests
Livello qualitativo
Confezionamento
pkg of 1 kit
tecniche
flow cytometry: suitable
applicazioni
cell analysis
detection
Metodo di rivelazione
fluorometric
Condizioni di spedizione
wet ice
Temperatura di conservazione
2-8°C
Caratteristiche e vantaggi
Detects apoptosis earlier in the process than DNA-based assays such as TUNEL
- Rapid labeling of cells. Cell staining takes only 10 minutes.
- No cell fixation or processing required, reducing the detection time and allowing the cells to be used for further study.
- 6-Carboxyfluorescein diacetate secondary dye is included with the kit to differentiate apoptotic cells from viable and necrotic cells.
Principio
Sigma′s Annexin V-Cy3 kit allows detection of annexin V bound to apoptotic cells by fluorescence microscopy. The Annexin V-Cy3 kit uses the dye Cy3.18 as the fluorochrome conjugated with annexin V. By microscopy, Cy3.18 fluoresces more brightly than the FITC conjugate. The kit includes the non-fluorescent compound 6-carboxyfluorescein diacetate (6-CFDA), which enters the cell and is hydrolyzed by the esterases present in living cells to the fluorescent compound 6-carboxyfluorescein, indicating that the cells are viable. This combination allows the differentiation among early apoptotic cells (annexin V positive, 6-CFDA positive), necrotic cells (annexin V positive, 6-CFDA negative), and viable cells (annexin V negative, 6-CFDA positive).
Note legali
Cy3 is a trademark of Cytiva
Solo come componenti del kit
N° Catalogo
Descrizione
- Annexin V Cy3.18 Conjugate from human placenta, buffered aqueous solution 10 μg
I componenti del kit sono disponibili anche separatamente
N° Catalogo
Descrizione
SDS
- C50416-Carboxyfluorescein diacetate 10 mgSDS
Codice della classe di stoccaggio
10 - Combustible liquids
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
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Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.
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