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Documenti fondamentali

A6103

Sigma-Aldrich

Aprotinin

recombinant, expressed in Nicotiana (tobacco), ≥5 TIU/mg protein, powder, ≥98% (SDS-PAGE), suitable for inhibition assay

Sinonimo/i:

BPTI, Basic pancreatic trypsin inhibitor, Kallikrein-trypsin inactivator, Kunitz protease inhibitor

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About This Item

Numero CE:
Codice UNSPSC:
12352202
NACRES:
NA.77

Nome del prodotto

Aprotinin Nicotiana tobacco, >= 5TIU/mg protein, >= 98 % SDS-PAGE | 9087-70-1, recombinant, expressed in Nicotiana (tobacco), ≥5 TIU/mg protein, ≥98% (SDS-PAGE)

Ricombinante

expressed in Nicotiana (tobacco)

Descrizione

recombinant form of thenative bovine-sequence aprotinin purified from plant tissue (tobacco)

Nome Commerciale

BioUltra

Saggio

≥98% (SDS-PAGE)

Stato

powder

Attività specifica

≥5 TIU/mg protein

PM

~_6.5 kDa

tecniche

inhibition assay: suitable

Solubilità

0.85% sodium chloride: 5 mg/mL

N° accesso UniProt

Temperatura di conservazione

2-8°C

Informazioni sul gene

cow ... PTI(404172)

Definizione di unità

One trypsin inhibitor unit (TIU) will decrease the activity of 2 trypsin units by 50% where one trypsin unit will hydrolyze 1.0 μmole of N-α-benzoyl-DL-arginine p-nitroanilide (BAPNA) per min at pH 7.8 at 25 °C.

Nota sulla preparazione

Contains no animal-derived components or impurities, and is manufactured by transient expression of the aprotinin message in RNA (+)-strand tobacco mosaic virus vectors propagated in non-transgenic Nicotiana plants. This is a recombinant form of the native, bovine-sequence aprotinin, which is traditionally isolated from bovine lung by methods involving fractional precipitation, gel filtration, and ion exchange chromatography. Unlike animal-derived aprotinin, this product is isolated and purified from plant tissue by proprietary methods.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)


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Protocolli

Enzymatic Assay of Aprotinin

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