A1970
Anti-VSV-Glycoprotein−Agarose antibody, Mouse monoclonal
clone P5D4, purified from hybridoma cell culture, PBS suspension
Sinonimo/i:
Monoclonal Anti-VSV Glycoprotein
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About This Item
Codice UNSPSC:
12352203
NACRES:
NA.56
Prodotti consigliati
Origine biologica
mouse
Livello qualitativo
Coniugato
agarose conjugate
Forma dell’anticorpo
purified immunoglobulin
Tipo di anticorpo
primary antibodies
Clone
P5D4, monoclonal
Forma fisica
PBS suspension
Classi chimiche degli analiti
proteins (VSV-G)
tecniche
immunoprecipitation (IP): suitable
protein purification: suitable
Isotipo
IgG1
Capacità
≥15 nmol/mL, resin binding capacity (VSV-G tagged fusion protein)
Condizioni di spedizione
wet ice
Temperatura di conservazione
2-8°C
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Descrizione generale
Anti-VSV-G Agarose Conjugate is the immunoglobulin fraction of Monoclonal Anti-VSV glycoprotein covalently linked to agarose.
Specificità
The antibody recognizes an epitope containing the five carboxy-terminal amino acids of VSV Glycoprotein. In infected cells, the antibody localizes the immature forms of VSV-G in the rough endoplasmic reticulum (RER) and in the cisternae of Golgi complex, as well as mature VSV-G at the cell surface and in the budding virus. The antibody does not stain the secreted form of VSV-G which lacks the membrane and the cytoplasmic domain. This antibody has been used for studies on the role of the cytoplasmic domain on newly-synthesized VSV-G during transfer to the plasma membrane and cell surface, using micro-injected antibody, immunoblotting, immunoprecipitation, immunocytochemistry and immunoelectron microscopy. The antibody has been used for the detection, immunoprecipitation and immunocytochemical staining of exogenously introduced constructs tagged with the carboxyl-terminus of VSV-G. This tag does not interfere with the function of the studied protein and can be specifically recognized by the P5D4 antibody without cross-reaction with any endogenous protein.
Immunogeno
synthetic peptide containing the 15 carboxy-terminal amino acids (497-511) of Vesicular Stomatitis Virus Glycoprotein (VSV-G), conjugated to KLH.
Applicazioni
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunoprecipitation (1 paper)
Immunoprecipitation (1 paper)
For immunoprecipitation and affinity purification of VSV-G tagged fusion proteins. The antibody recognizes an epitope containing the five carboxy-terminal amino acids of VSV Glycoprotein.
Stato fisico
Supplied as a suspension (1:1,v/v) of beaded agarose in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as preservative
Nota sulla preparazione
Prepared using cyanogen bromide-activated agarose.
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Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
nwg
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
Yael Katan-Khaykovich et al.
Proceedings of the National Academy of Sciences of the United States of America, 108(4), 1296-1301 (2011-01-12)
Nucleosome deposition occurs on newly synthesized DNA during DNA replication and on transcriptionally active genes via nucleosome-remodeling complexes recruited by activator proteins and elongating RNA polymerase II. It has been long believed that histone deposition involves stable H3-H4 tetramers, such
Bridget R Kulasekara et al.
eLife, 2, e01402-e01402 (2013-12-19)
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The Journal of biological chemistry, 285(45), 34566-34578 (2010-08-26)
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Rui Chen et al.
The Journal of biological chemistry, 287(36), 30800-30811 (2012-07-19)
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