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Key Documents

A0162

Sigma-Aldrich

Anti-Mouse Polyvalent Immunoglobulins (G,A,M)−Alkaline Phosphatase antibody produced in goat

affinity isolated antibody, buffered aqueous solution

Sinonimo/i:

Goat Anti-Mouse Polyvalent Immunoglobulins (G,A,M)−AP

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About This Item

Numero MDL:
Codice UNSPSC:
12352203
NACRES:
NA.46

Origine biologica

goat

Coniugato

alkaline phosphatase conjugate

Forma dell’anticorpo

affinity isolated antibody

Tipo di anticorpo

secondary antibodies

Clone

polyclonal

Forma fisica

buffered aqueous solution

Reattività contro le specie

mouse

tecniche

direct ELISA: 1:3,000 using IgG, IgA, IgM
western blot: 1:50 dilution

Condizioni di spedizione

wet ice

Temperatura di conservazione

2-8°C

modifica post-traduzionali bersaglio

unmodified

Descrizione generale

Immunoglobulins are proteins produced by B cells in response to antigen and regulate response to bacteria and viruses. IgG is known to regulate complement fixation and placental transport. IgA has a crucial role in mucosal immunity as it restricts pathogens from entering the mucosal membrane. IgM is the largest antibody having a pentameric structure which modulates polyreactivity and removes apoptotic cells.

Applicazioni

Alkaline-phosphatase-conjugated anti-mouse polyvalent immunoglobulin was used to detect antibodies from hybridomas by indirect ELISA. The antibody was incubated in microtiter plates for 1 hour at 37°C and detected using p-nitrophenyl phosphate substrate (Sigma). Alkaline-phosphatase-conjugated anti-mouse polyvalent immunoglobulin was used to detect the reactivity of fungal cytoplasmic protein with proteins from the serum of mice infected with yeast-form Y cells by western blot analysis. The antibody was used at a 1:50 dilution.

Stato fisico

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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Nature communications, 9(1), 4215-4215 (2018-10-13)
Infectious diseases are mostly explored using reductionist approaches despite repeated evidence showing them to be strongly influenced by numerous interacting host and environmental factors. Many diseases with a complex aetiology therefore remain misunderstood. By developing a holistic approach to tackle
C Tartera et al.
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Monoclonal antibodies provide a rapid and specific means of direct detection of microorganisms in water and food samples. However, monoclonal antibodies specific for some bacteria are difficult to obtain; a good example of such a bacterium is Escherichia coli. Gnotobiotic
Carla Bromuro et al.
Infection and immunity, 70(10), 5462-5470 (2002-09-14)
Mice immunized with heat-inactivated, whole yeast-form cells (Y cells) of Candida albicans developed intense, specific humoral and cell-mediated immune responses. However, they were modestly protected against a lethal challenge by the fungus, and their sera did not confer passive protection
Jonas Nilsson et al.
Glycoconjugate journal, 26(9), 1171-1180 (2009-04-24)
Noroviruses and norovirus virus-like particles (VLPs) exhibit strain specific patterns in their binding to ABH and Lewis histo-blood group antigens. In this study we demonstrate for the first time specific binding of Norwalk virus VLPs to type 1 and type
David A Six et al.
Antimicrobial agents and chemotherapy, 58(1), 153-161 (2013-10-23)
The β-acetoacetyl-acyl carrier protein synthase FabY is a key enzyme in the initiation of fatty acid biosynthesis in Pseudomonas aeruginosa. Deletion of fabY results in an increased susceptibility of P. aeruginosa in vitro to a number of antibiotics, including vancomycin

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