59767

Chlorophenol Red-β-D-galactopyranoside

β-galactosidase, ≥90% (HPLC), powder or crystals

• Sinonimo/i: CPRG, Chlorophenol Red-β-D-galactoside
• Formula empirica (notazione di Hill): C₂₅H₂₂Cl₂O₁₀S
• Numero CAS: 99792-79-7
• Peso molecolare: 585.41
• Numero MDL: MFCD05863726
• Codice UNSPSC: 12352204
• ID PubChem: 57651696
• NACRES: NA.83

Proprietà

• product name: Chlorophenol Red-β-D-galactopyranoside, ≥90% (HPLC)
• Saggio: ≥90% (HPLC)
• Forma fisica: powder or crystals
• Solubilità: water: 20 mg/mL, clear, orange to very deep red
• Temperatura di conservazione: −20°C
• Stringa SMILE: OC[C@H]1O[C@@H](Oc2ccc(cc2Cl)C(=C3/C=CC(=O)C(Cl)=C3)\c4ccccc4S(O)(=O)=O)[C@H](O)[C@@H](O)[C@H]1O
• InChI: 1S/C25H22Cl2O10S/c26-15-9-12(5-7-17(15)29)21(14-3-1-2-4-20(14)38(33,34)35)13-6-8-18(16(27)10-13)36-25-24(32)23(31)22(30)19(11-28)37-25/h1-10,19,22-25,28,30-32H,11H2,(H,33,34,35)/b21-12-/t19-,22+,23+,24-,25-/m1/s1; YOUWVNCQJOMMEU-AETRGLENSA-N

Descrizione

Descrizione generale

Chlorophenol red-β-D-galactopyranoside (CPRG) is a long-wavelength yellow dye commonly used for colorimetric assays. It serves as a substrate for β-galactosidase (β-gal) which hydrolyzes CPRG to form chlorophenol red (purple colored). This product is quantified by measuring its absorbance at 570 nm. CPRG is used to identify fecal coliform bacteria. It is about 10 times more sensitive than other chromogenic agents such as o-nitrophenyl-β-galactoside (ONPG). CPRG also has a high rapid turnover.

Applicazioni

Chlorophenol Red-β-D-galactopyranoside has been used as a substrate for β-galactosidase (β-gal):

• in paper-based cell-free protein expression system for target RNA detection
• in colorimetric detection of multiple clinically relevant mutations using a lacZ output gene
• in colorimetric assay for bacteria quantification

Altre note

Measures the expression levels of β-galactosidase in transfected cells. The lacZ gene is a popular reporter gene in transfection reactions because its product, β-galactosidase, is a very stable enzyme.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, type N95 (US)