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57620

Sigma-Aldrich

Inulinase from Aspergillus niger

lyophilized, powder, brown-gray, ~25 U/mg

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About This Item

Numero CAS:
Classificazione EC (Enzyme Commission):
Numero CE:
Numero MDL:
Codice UNSPSC:
12352204
NACRES:
NA.54

Origine biologica

Aspergillus niger

Livello qualitativo

Stato

powder

Qualità

lyophilized

Attività specifica

~25 U/mg

Colore

brown-gray

Temperatura di conservazione

2-8°C

Descrizione generale

Inulinases are classified as exoinulinases and endoinulinases.

Applicazioni

Inulinase from Aspergillus niger has been used as a glycoside hydrolase (GH) to study its potential to disperse bacteria (P. aeruginosa and S. aureus) from microbial biofilms using a polymicrobial well-plate model.

Azioni biochim/fisiol

Inulinase is used in high-fructose syrup, which has therapeutic applications. Inulinase is reported to have high activity toward sucrose.
Inulinase hydrolyses inulin to produce oligosaccharides and liberate fructose. It also splits the terminal fructose units into sucrose and raffinose.

Definizione di unità

1 U corresponds to the amount of enzyme which releases 1 μmol of reducing sugar (measured as fructose) per minute at pH 4.1 and 37°C from inulin

Altre note

Characterization

Pittogrammi

Health hazard

Avvertenze

Danger

Indicazioni di pericolo

Consigli di prudenza

Classi di pericolo

Resp. Sens. 1

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)


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R. Azhari et al.
Biotechnology and Applied Biochemistry, 11, 105-105 (1989)
Gonçalo L M Santa et al.
Applied biochemistry and biotechnology, 165(1), 1-12 (2011-03-30)
The present work aims to provide the basic characterization of sol-gel immobilized inulinase, a biocatalyst configuration yet unexploited, using as model system the hydrolysis of inulin to fructose. Porous xerogel particles with dimensions in slight excess of 10 μm were obtained
Pierre Delanaye et al.
Clinica chimica acta; international journal of clinical chemistry, 413(5-6), 556-560 (2011-12-14)
Among issues susceptible to hamper a reliable measurement of inulin clearance, those regarding the dosage of inulin are largely neglected. We have compared the analytical performances of 2 commonly used methods of inulin dosage (one "acid" and one "enzymatic" method)
Wen Wan et al.
Applied microbiology and biotechnology, 91(3), 789-798 (2011-06-10)
Easy and low-cost protein purification methods for the mass production of commonly used enzymes that play important roles in biotechnology are in high demand. In this study, we developed a fast, low-cost recombinant protein purification system in the methylotrophic yeast
Takahiro Rikita et al.
Analytical biochemistry, 426(1), 24-26 (2012-04-10)
Inulin is a polysaccharide composed mainly of D-fructose units and is the most reliable indicator of the glomerular filtration rate. We have proposed an inulin detection method that involves the hydrolysis of inulin to D-fructose using inulinase and the selective

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