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50492

Sigma-Aldrich

Atto Rho101 NHS ester

BioReagent, suitable for fluorescence

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About This Item

Codice UNSPSC:
12352108
NACRES:
NA.32

Nome Commerciale

BioReagent

Saggio

90% (Coupling efficiency with benzylamine, HPLC)

Forma fisica

solid

Produttore/marchio commerciale

ATTO-TEC GmbH

Trasmittanza

254 nm
586 nm

Fluorescenza

λex 586 nm; λem 610 nm

λ

in ethanol (0.1% trifluoroacetic acid)

Compatibilità

suitable for fluorescence

Temperatura di conservazione

−20°C

Applicazioni

Atto Rho101 NHS ester is a derivative of the well-known dye Rhodamine 101 for coupling to bio-molecules such as DNA, RNA or proteins. Characteristic features of the label are strong absorption, extraordinarily high fluorescence quantum yield, and high thermal and photo-stability. The dye is moderately hydrophilic. ATTO Rho101 is a cationic dye. After coupling to a substrate the dye carries a net electrical charge of +1.

Codice della classe di stoccaggio

11 - Combustible Solids

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

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Kakishi Uno et al.
Journal of the American Chemical Society, 133(34), 13558-13564 (2011-08-09)
Photoswitchable or photoactivatable fluorescent dyes are potentially applicable to ultrahigh density optical memory media as well as super-resolution fluorescence imaging when the dyes are highly fluorescent and have large absorption coefficients. Here, we report on highly fluorescent photochromic dyes, which
Li-lin Jiang et al.
Guang pu xue yu guang pu fen xi = Guang pu, 31(5), 1348-1351 (2011-08-02)
The absorption, fluorescence and time-resolved fluorescence spectra of Rhodamine 101 dye in both methanol and acidic methanol solutions were measured. The authors achieved the characteristic information of the absorption and fluorescence spectra, and obtained the S1 lifetimes. The authors assigned
Knut Rurack et al.
Analytical chemistry, 83(4), 1232-1242 (2011-01-22)
The determination of the fluorescence quantum yields (QY, Φ(f)) of a series of fluorescent dyes that span the absorption/excitation and emission ranges of 520-900 and 600-1000 nm is reported. The dyes encompass commercially available rhodamine 101 (Rh-101, Φ(f) = 0.913)
Christian Würth et al.
Analytical chemistry, 84(3), 1345-1352 (2012-01-17)
There is an increasing interest in chromophores absorbing and emitting in the near-infrared (NIR) spectral region, e.g., for applications as fluorescent reporters for optical imaging techniques and hence, in reliable methods for the characterization of their signal-relevant properties like the

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