72464
Atto 565 NHS ester
BioReagent, suitable for fluorescence, ≥90% (HPLC)
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About This Item
Formula empirica (notazione di Hill):
C35H34ClN3O11
Peso molecolare:
708.11
Numero MDL:
Codice UNSPSC:
12352108
NACRES:
NA.32
Prodotti consigliati
Nome Commerciale
BioReagent
Livello qualitativo
Saggio
≥90% (HPLC)
≥90% (degree of coupling)
Stato
powder
Produttore/marchio commerciale
ATTO-TEC GmbH
Compatibilità
suitable for fluorescence
Metodo di rivelazione
fluorometric
Temperatura di conservazione
−20°C
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Descrizione generale
Atto 565 NHS ester is a commonly used amine-reactive reagent, provided by ATTO-TEC. It is widely used in protein labeling and readily reacts with compounds containing amino groups, forming a chemically stable amide bond between the dye, e.g., a protein. The optimum pH-range for NHS-ester coupling is pH 8.0 – 9.0. At this pH amino groups of proteins, i.e., the ε-amino groups of lysines are unprotonated to a degree sufficiently high for fast coupling.
Caratteristiche e vantaggi
- Strong absorption
- High fluorescence quantum yield
- High thermal and photo-stability
Altre note
Fluorescence of bioconjugates; employed in fluorescence resonance energy transfer, FRET, experiments.
Note legali
This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, type N95 (US)
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Fluorescent Labeling of Peptides
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