47671

Formamide

BioUltra, for molecular biology, ≥99.5% (T)

• Sinonimo/i: Amide C₁, Ammide formica
• Formula condensata: HCONH₂
• Numero CAS: 75-12-7
• Peso molecolare: 45.04
• Beilstein: 505995
• Numero CE: 200-842-0
• Numero MDL: MFCD00007941
• Codice UNSPSC: 12352111
• ID PubChem: 57651075
• NACRES: NA.25

Proprietà

• Grado: Molecular Biology; for molecular biology
• Densità del vapore: 1.55 (vs air)
• Tensione di vapore: 0.08 mmHg ( 20 °C); 30 mmHg ( 129 °C)
• Nome Commerciale: BioUltra
• Saggio: ≥99.5% (T)
• Stato: liquid
• Temp. autoaccensione: 932 °F
• tecniche: FISH: suitable; hybridization: suitable; protein extraction: suitable
• Impurezze: DNases, none detected; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected
• Residuo alla calcinazione: ≤0.1%
• Indice di rifrazione: n20/D 1.447 (lit.); n20/D 1.447
• pH: 7-9 (25 °C, 1 M in H₂O)
• P. ebollizione: 210 °C (lit.)
• Punto di fusione: 2-3 °C (lit.)
• Solubilità: H₂O: 10 M at 20 °C, clear, colorless
• Densità: 1.134 g/mL at 25 °C (lit.)
• Anioni in tracce: chloride (Cl^-): ≤1 mg/kg; sulfate (SO₄^2-): ≤10 mg/kg
• Cationi in tracce: Ag: ≤5 mg/kg; Al: ≤1 mg/kg; As: ≤0.1 mg/kg; Ba: ≤1 mg/kg; Bi: ≤1 mg/kg; Ca: ≤5 mg/kg; Cd: ≤1 mg/kg; Co: ≤1 mg/kg; Cr: ≤1 mg/kg; Cu: ≤1 mg/kg; Fe: ≤1 mg/kg; K: ≤20 mg/kg; Li: ≤1 mg/kg; Mg: ≤1 mg/kg; Mn: ≤1 mg/kg; Mo: ≤1 mg/kg; NH₄⁺: ≤1000 mg/kg; Na: ≤20 mg/kg; Ni: ≤1 mg/kg; Pb: ≤1 mg/kg; Sr: ≤1 mg/kg; Tl: ≤5 mg/kg; Zn: ≤1 mg/kg
• Stringa SMILE: NC=O
• λ: 10 M in H₂O
• Assorbanza UV: λ: 260 nm A_max: ≤0.4; λ: 280 nm A_max: ≤0.1
• applicazioni: genomic analysis
• InChI: 1S/CH3NO/c2-1-3/h1H,(H2,2,3); ZHNUHDYFZUAESO-UHFFFAOYSA-N

Descrizione

Descrizione generale

Formamide, also referred to as Carbamaldehyde, is an amide compound derived from formic acid. It plays a crucial role in biochemical research, particularly in denaturation and renaturation of nucleic acids (RNA and DNA). This includes applications like hybridization, sequencing gel electrophoresis, and electron microscopy. In molecular biology, BioUltra Formamide finds extensive use in various applications such as nucleic acid hybridization buffers, genetic fingerprinting, Southern and Northern blotting, as well as YAC screening. It typically functions by lowering the melting temperature of nucleic acid hybrids, facilitating their manipulation, and can enhance denaturation conditions in sequencing gels for more robust results.

Applicazioni

La formamide destabilizza le doppie eliche dell′acido nucleico e può essere usata, di solito, a una concentrazione del 50%, in protocolli di ibridazione che richiedono temperature di ibridazione inferiori.

Formamide has been used:

• as a component in the hybridization buffer for in situ hybridization as a component of solution X to wash the slides to remove the coverslips
• in the preparation of denaturation solution for spectral karyotyping (SKY) analysis
• in the preparation of 1% formaldehyde solution for the pretreatment of slides for comparative genomic hybridization (CGH)
• to prepare pre-hybridization buffer I, II, III, IV, and hybridization buffer I, II, and III for RNA fluorescence in situ hybridization (RNA-FISH)
• as an ionizing solvent in the production of formic esters and vitamin, a softener for paper, in animal glues, and water-soluble gums, in molecular biology as a denaturing agent

Azioni biochim/fisiol

Formamide serves as a good solvent for polar organic and inorganic compounds with potential catalytic activity in prebiotic processes due to its high dielectric constant.

Caratteristiche e vantaggi

• Versatile and adaptable for a wide variety of laboratory and research applications
• BioUltra Grade for your Molecular Biology, Cell Biology and Biochemical research
• Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
• Free from DNase, RNase, NICKase and proteaseTested for trace levels of Anions and Cations

Altre note

For additional information on our range of Biochemicals, please complete this form.

For the preparation of formamide denaturing polyacrylamide gels; In situ hybridization on human chromosomes

Informazioni sulla sicurezza

• Pittogrammi: GHS08
• Avvertenze: Danger
• Indicazioni di pericolo: H351,H360D,H373
• Consigli di prudenza: P202 - P260 - P280 - P308 + P313 - P405 - P501
• Classi di pericolo: Carc. 2 - Repr. 1B - STOT RE 2 Oral
• Organi bersaglio: Blood
• Codice della classe di stoccaggio: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): 305.6 °F
• Punto d’infiammabilità (°C): 152 °C
• Dispositivi di protezione individuale: Eyeshields, Gloves, type ABEK (EN14387) respirator filter