35848

2′,7′-Dichlorofluorescein

BioReagent, suitable for fluorescence, ≥90% (T)

• Sinonimo/i: DCF, Dichlorofluorescein
• Formula empirica (notazione di Hill): C₂₀H₁₀Cl₂O₅
• Numero CAS: 76-54-0
• Peso molecolare: 401.20
• Beilstein: 58009
• Numero CE: 200-968-6
• Numero MDL: MFCD00005047
• Codice UNSPSC: 12171500
• ID PubChem: 57649439
• NACRES: NA.32

Proprietà

• Nome Commerciale: BioReagent
• Saggio: ≥90% (T)
• Stato: solid
• Punto di fusione: 280 °C (dec.) (lit.)
• Fluorescenza: λ_ex 504 nm; λ_em 529 nm in 0.1 M Tris pH 8.0
• Compatibilità: suitable for fluorescence
• Stringa SMILE: Oc1cc2Oc3cc(O)c(Cl)cc3C4(OC(=O)c5ccccc45)c2cc1Cl
• InChI: 1S/C20H10Cl2O5/c21-13-5-11-17(7-15(13)23)26-18-8-16(24)14(22)6-12(18)20(11)10-4-2-1-3-9(10)19(25)27-20/h1-8,23-24H; VFNKZQNIXUFLBC-UHFFFAOYSA-N

Descrizione

Descrizione generale

2′,7′-Dichlorofluorescein, also known as DCF is a fluorescent dye. The DCF fluorescence excitation is at 498nm with emission at 522nm. The lipophilic non-fluorescent Dcfh-DA readily crosses the cell membrane through passive diffusion followed by deacetylation. The deacylated product is an oxidant sensitive 2′,7′-dichlorofluorescein (DCHF). DCHF is oxidized later to form highly fluorescent DCF, which is measured.

Applicazioni

2′,7′-Dichlorofluorescein is used as fluorescent probe to measure ROS (Reactive Oxygen Species) for biological studies. It may also be used as a dip reagent for the fluorometric determination of vicinal diols, glycosides, and phenols.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: dust mask type N95 (US), Eyeshields, Gloves