08124
Isoamylase
ammonium sulfate suspension, ≥10,000,000 units/mg protein
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About This Item
Numero MDL:
Codice UNSPSC:
12352204
NACRES:
NA.54
Prodotti consigliati
Stato
ammonium sulfate suspension
Attività specifica
≥10,000,000 units/mg protein
Temperatura di conservazione
2-8°C
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Applicazioni
Isoamylase from Pseudomonas sp. has been used in starch debranching and to measure the chain-length distributions.
Azioni biochim/fisiol
Pseudomonas amyloderamosa is considered a popular source of isoamylase. In the presence of a surfactant, this isoamylase produces a series of maltooligosaccharides. Immobilized isoamylase makes it applicable with a polysaccharide matrix composed of agarose, cellulose, and raw corn starch.
Catalyzes the hydrolysis of (1-6)-α-D-glucosidic branch linkages in glycogen, amylopectin and their β-limit dextrins.
Definizione di unità
One unit causes an increase in A610 of 0.1 in 1 hour using a homogenized solution of rice starch as a substrate (pH 3.5, 40°C)
Risultati analitici
≥10 MU/mg protein
Avvertenze
Danger
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2
Codice della classe di stoccaggio
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
Classe di pericolosità dell'acqua (WGK)
WGK 2
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
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Glycogen, highly branched (1-->4)(1-->6)-linked alpha-d-glucan, can be extracted from natural sources such as animal tissues or shellfish (natural source glycogen, NSG). Glycogen can also be synthesized in vitro from glucose-1-phosphate using the cooperative action of alpha-glucan phosphorylase (GP, EC 2.4.1.1)
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Starch debranching is fundamental for understanding the structure-function relationships of starch. In this paper, atomic force microscopy (AFM) was used to investigate potato starch by isoamylase [EC 3.2.1.68] debranching at nanometer scale. The hydrolysates were separated by gel-permeation chromatography and
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