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M8802

Millipore

Malonate broth

pH 6.7±0.2 (25 °C), NutriSelect® Plus, suitable for microbiology

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About This Item

Codice UNSPSC:
41171607
NACRES:
NA.85

Sterilità

non-sterile

Livello qualitativo

200

Forma fisica

powder

Produttore/marchio commerciale

NutriSelect® Plus

tecniche

microbe id | utilization test: suitable
microbiological culture: suitable

pH

6.7±0.2 (25 °C)

applicazioni

food and beverages
water monitoring
microbiology

Compatibilità

Klebsiella spp.
Salmonella spp.
nonselective and differential for Enterobacter spp.
nonselective and differential for Escherichia coli
nonselective and differential for coliforms

Descrizione generale

Malonate broth is recommended for the differentiation of Enterobacter and Escherichia.

Applicazioni

Malonate broth has been used to evaluate the utilization of malonate by Enterobacter sakazakii. It has also been used to differentiate between Cronobacter sakazakii and Cronobacter malonaticus isolates.

Componenti

Components (g/L)
Ammonium sulfate, 2.00
Dipotassium phosphate, 0.60
Monopotassium phosphate, 0.40
Sodium chloride, 2.00
Sodium malonate, 3.00
Bromo thymol blue, 0.025

Nota sulla preparazione

Suspend 8 g of Malonate Broth in 1000 ml of distilled water. Dispense and sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes. Avoid the addition of carbon and nitrogen from other sources.

Nota a piè di pagina

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Note legali

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

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Certificati d'analisi (COA)

Lot/Batch Number
BCCG1477
BCCC9735
BCBX0472
BCBW3879
BCBQ6504V

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Sodium malonate dibasic ≥97.0% (NT)

Sigma-Aldrich

63409

Sodium malonate dibasic

Simmons Citrate Agar NutriSelect® Basic, suitable for microbiology

Millipore

85463

Simmons Citrate Agar

Brodo infuso cuore-cervello GranuCult® ISO 6888, To support the growth of fastidious and non-fastidious microorganisms

Millipore

1.10493

Brodo infuso cuore-cervello GranuCult®

L-lisina ≥98% (TLC)

Sigma-Aldrich

L5501

L-lisina

L-Ornithine monohydrochloride ≥99%

Sigma-Aldrich

O2375

L-Ornithine monohydrochloride

Dimethyl malonate 98%

Sigma-Aldrich

136441

Dimethyl malonate

Malonic acid ReagentPlus®, 99%

Sigma-Aldrich

M1296

Malonic acid

Brodo LB con agar (Lennox) Highly-referenced microbial growth powder medium with Agar, low salt, suitable for salt-sensitive E. coli culture.

Sigma-Aldrich

L2897

Brodo LB con agar (Lennox)

Carol Iversen et al.
BMC evolutionary biology, 7, 64-64 (2007-04-19)
Enterobacter sakazakii is an opportunistic pathogen that can cause infections such as necrotizing enterocolitis, bacteraemia, meningitis and brain abscess/lesions. When the species was defined in 1980, 15 biogroups were described and it was suggested that these could represent multiple species.
Amy Strydom et al.
Food microbiology, 28(8), 1472-1477 (2011-09-20)
Members of the genus Cronobacter are opportunistic pathogens associated with life-threatening infections in immuno-compromised individuals. Polyphasic analysis has facilitated the classification of the novel genus Cronobacter containing five species. However, since this recent reclassification there are not many identification methods
Ewing, W., et al.
Publ. Hlth. Lab., 15, 153-153 (1957)
MacFaddin, J
Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, 1 (1985)

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