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B9431

Sigma-Aldrich

Biotin-4-Fluorescein

≥95% purity (HPLC), powder

Sinonimo/i:

5-(6)-[2-(biotinoyl)aminoethylaminocarbonyl] fluorescein, B4F, Biotin (5-fluorescein) conjugate

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About This Item

Formula empirica (notazione di Hill):
C33H32N4O8S
Peso molecolare:
644.69
Codice UNSPSC:
12171500
eCl@ss:
34058011
ID PubChem:
329773319
NACRES:
NA.47

product name

Biotin-4-Fluorescein, ≥95.0% (HPLC)

Livello qualitativo

100

Saggio

≥95.0% (HPLC)

Forma fisica

powder

PM

644.71

Colore

light orange to very dark orange
light yellow to very dark yellow

ε (coefficiente d’estinzione)

≥70 at 491-497 nm in phosphate buffer at 0.05 M

applicazioni

diagnostic assay manufacturing
hematology
histology

Temperatura di conservazione

room temp

Stringa SMILE

OC(=O)c1cc(ccc1C2=C3C=CC(=O)C=C3Oc4ccc(O)cc24)C(=O)NCCNC(=O)CCCC[C@@H]5SC[C@@H]6NC(=O)N[C@H]56

Applicazioni

Biotin-4-Fluorescein has been used for the labeling of avidin to study the changes in protein binding capability after carbon negative-ion implantation into avidin. It has been used to study the binding affinity of streptavidin towards biotin.
Useful for quantitating biotin binding sites. This reagent binds to avidin more quickly than conventional fluorescein biotin.

Azioni biochim/fisiol

Biotin-fluorescein is mainly used for the assay of biotin-interaction sites in avidin and streptavidin. Binding of biotin-4-fluorescein results in strong quenching, which is used for measuring the concentration of (strept)avidin.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

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Certificati d'analisi (COA)

Lot/Batch Number
MKCP6327
MKCP0536
MKCM9151
MKCL7119
MKCK9990

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The Journal of cell biology, 187(1), 53-60 (2009-09-30)
Unconventional myosins interact with the dense cortical actin network during processes such as membrane trafficking, cell migration, and mechanotransduction. Our understanding of unconventional myosin function is derived largely from assays that examine the interaction of a single myosin with a
Cell-Adhesion Patterning by using Carbon Negative-Ion Implantation into Albumin and Avidin Layers on Polystyrene.
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Rapid estimation of avidin and streptavidin by fluorescence quenching or fluorescence polarization.
Kada G, et al.
Biochimica et Biophysica Acta, 1427, 44-48 (1999)
Nathaniel G Hentz et al.
Journal of laboratory automation, 19(2), 153-162 (2013-09-14)
This study illustrates how optimization of both liquid-handling accuracy and precision is critical to assay performance. The study was designed to examine (1) liquid-handling performance and (2) the effect of liquid-handling variability on two types of in vitro biochemical assays

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